对CTAB法提取菠菜基因组DNA实验的优化设计  

Conditions Research on CTAB Extraction of Spinach Genome DNA

作　　者：李岩 于海琳 马晓彤 杨茜[1] 张海燕[1] LI Yan;YU Hai-lin;MA Xiao-tong;YANG Qian;ZHANG Hai-yan(Heze Medical College,Heze 274000,China)

机构地区：[1]菏泽医学专科学校,山东菏泽274000

出　　处：《化工管理》2023年第14期15-17,共3页Chemical Engineering Management

基　　金：山东省教育发展研究微课题(No.FJ021);山东省医药卫生科技发展计划项目(No.2018WS494);山东省高等学校科技计划项目(No.J18KA295)。

摘　　要：CTAB法是一种经典的提取植物DNA的方法,但传统的提取方法存在RNA污染、DNA提纯率不高等问题。基于这些问题,文章对实验步骤进行了优化,并将使用改良方法提取的菠菜叶片DNA通过紫外分光光度法进行检测。结果表明,使用改良方法提取的DNA其浓度及纯度显著提高,有效提升了实验教学的质量和效率。The methods of CTAB is a classical method for extracting plant DNA,but the traditional extraction method has RNA pollution and low DNA purif ication rate.Based on these problems,the experimental steps were optimized,and the spinach leaf DNA extracted using a modif ied method was detected by UV spectrophotometry.The results showed that the concentration and purity of the DNA extracted with the improved method were improved signif icantly,which effectively improved the quality and eff iciency of the experimental teaching.

关 键 词：菠菜 CTAB法 DNA提取 实验教学 

分 类 号：G633[文化科学—教育学]