宏基因组学技术在蔬菜发酵中的应用  

Application of Metagenomics Technology in Vegetable Fermentation

作　　者：张令 罗根 杨岩 王泳俨 张美霞[2] ZHANG Ling;LUO Gen;YANG Yan;WANG Yongyan;ZHANG Meixia(College of Biology and Food Engineering,Chongqing Three Gorges University,Chongqing 404120,China;College of Landscape Architecture and Life Sciences,Chongqing University of Arts and Science,Chongqing 402160,China)

机构地区：[1]重庆三峡学院生物与食品工程学院,重庆404120 [2]重庆文理学院园林与生命科学学院,重庆402160

出　　处：《农产品加工》2023年第8期71-75,80,共6页Farm Products Processing

基　　金：重庆市科技局自然科学基金(面上项目)(CSTC2020JCYJ-MSXMX0958);重庆市大学生创新创业训练计划项目(S202110642021)。

摘　　要：发酵蔬菜历史悠久、风味独特,深受广大人民群众的喜爱。蔬菜发酵中微生物群落结构多样性及不同代谢途径对产品的风味、品质与安全具有重要的影响。目前,高通量测序技术以通量高、准确率强等优势逐渐成为蔬菜发酵微生物群落研究中的重要工具。以高通量测序技术的宏基因组技术为起点,综述了宏组学技术对四川泡菜、东北酸菜和东北辣白菜不同腌制环境中微生物群落多样性、结构和潜在基因功能的研究进展,以及各地区微生物菌群与品质之间的相关性分析,为宏基因组学技术在蔬菜发酵行业中微生物的检测和产品品质的优化提供理论支撑。Fermented vegetables have a long history and unique flavor,which are deeply loved by the masses.The diversity of microbial community structure and different metabolic pathways in vegetable fermentation have important effects on the flavor,quality and safety of products.At present,high-throughput sequencing technology has gradually become an important tool in the study of vegetable fermentation microbial communities due to its high throughput and high accuracy.Based on the metagenomic technology of high-throughput sequencing technology,this paper reviewed the research progress of metagenomic technology on the diversity,structure and potential gene function of microbial communities in different pickled environments of Sichuan pickles,Northeast sauerkraut and Northeast spicy cabbage,as well as the correlation analysis between microbial flora and quality in various regions,so as to provide theoretical support for the detection of microorganisms and the optimization of product quality in vegetable fermentation industry by metagenomic technology.

关 键 词：发酵蔬菜 高通量测序 宏基因组学技术 微生物群落 

分 类 号：TS201.3[轻工技术与工程—食品科学]