瓦尼桑黄多酚类化合物纯化及抗氧化活性  

Purification and antioxidant activities of polyphenolic compounds from Sanghuangporus vaninii

作　　者：张洋洋 张作法[1] 宋婷婷[1] 蔡为明[1] 吕国英[1] ZHANG Yangyang;ZHANG Zuofa;SONG Tingting;CAI Weiming;LÜGuoying(Institute of Horticulture,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,Zhejiang,China)

机构地区：[1]浙江省农业科学院园艺研究所,浙江杭州310021

出　　处：《菌物学报》2023年第4期973-983,共11页Mycosystema

基　　金：浙江省农业新品种选育重大科技专项(2021C02073);海宁市科技计划农业项目(2022029)。

摘　　要：瓦尼桑黄中含有较多的酚类化合物,多酚类化合物具有较高的抗氧化活性。本研究采用深共熔溶剂(deep eutectic solvent,DES)提取多酚类化合物,通过单因素试验确定大孔树脂纯化桑黄多酚的最佳工艺参数。实验结果表明:HPD-100大孔树脂纯化桑黄多酚的效果最好,其静态吸附-解吸最佳参数为:吸附时间为4 h,上样浓度10 mg/mL、上样液pH 4.0、解吸乙醇浓度为70%;动态吸附-解吸最佳参数为:上样量100mL、洗脱量110mL。在最佳条件下桑黄子实体多酚的纯度从14.56%提高到33.81%,纯化后的多酚具有良好的抗氧化活性能力。DPPH和ABTS自由基清除能力分别为92.75%和93.03%,对牛血清蛋白氧化损伤保护效果良好,能有效抑制L929细胞的衰老。Sanghuangporus vaninii contains many phenolic compounds,and the polyphenols show high antioxidant activities.In this study,deep eutectic solvent(DES)was used for extraction of polyphenolic compounds of S.vaninii,and the optimal process parameters for the purification using macroporous resin were determined by a single-factor test.The experimental results showed that HPD-100 macroporous resin gave the best results for purification of S.vaninii polyphenols,and the optimal parameters for static adsorption-desorption were adsorption time of 4 h,loading solution concentration of 10 mg/mL,pH value of 4.0 and desorption ethanol concentration of 70%;the optimal parameters for dynamic adsorption-desorption were loading sample volume of 100 mL and elution volume of 110 mL.The purity of polyphenols increased from 14.56%to 33.81%,and the purified polyphenols had good antioxidant activities.DPPH and ABTS free radical scavenging capacities were 92.75%and 93.03%respectively,effectively preventing oxidative damage to bovine serum proteins and inhibiting the senescence of L929 cells.

关 键 词：瓦尼桑黄 大孔树脂 多酚 抗氧化活性 蛋白氧化损伤 细胞抗衰老 

分 类 号：R285[医药卫生—中药学]