山樱花品种间花色差异的代谢组学研究  

Metabonomics study on flower color differences of Prunus serrulata cultivars

作　　者：叶琦 潘靖宜 张敏[1,2] 伊贤贵 王贤荣[1,2] 李蒙 YE Qi;PAN Jingyi;ZHANG Min;YI Xiangui;WANG Xianrong;LI Meng(Co-Innovation Center for Sustainable Forestry in Southern China,Nanjing 210037,China;College of Biology and Environment,Nanjing Forestry University,Nanjing 210037,China)

机构地区：[1]南京林业大学南方现代林业协同创新中心,南京210037 [2]南京林业大学生物与环境学院,南京210037

出　　处：《广西植物》2023年第4期732-740,共9页Guihaia

基　　金：江苏省重点研发计划(现代农业)项目(BY2018067)。

摘　　要：山樱花是世界著名的观花类植物,花色是其最重要的观赏特征。为探究影响山樱花品种间花色差异的代谢通路及关键代谢产物变化,该文利用LC-MS/MS技术对白色、绿色和粉色的山樱花品种进行花青素靶向代谢组学比较分析。结果表明:(1)共检测到42种花青素物质,主要包含矮牵牛素、飞燕草素、黄酮类化合物、锦葵色素、芍药花素、矢车菊素、天竺葵素和原花青素8种物质。(2)差异代谢花青素25种,包括11种下调、14种上调,其中有7种花青素在粉色花瓣中显著富集。(3)KEGG通路注释发现差异代谢物在花青素生物合成通路中显著富集,结合聚类结果发现矮牵牛素-3-O-葡萄糖苷是山樱花品种间花色差异产生的关键代谢物。该研究揭示了山樱花花色差异的代谢机理,为后续山樱花花色分子调控机制研究提供了一定的理论依据,也为新品种花色改良和选育提供了一定的科学参考。Prunus serrulata is a famous ornamental plant worldwide.Flower color is the most prominent trait of cherry.In order to analyze the different pathways and key metabolite change in flower color among P.serrulata cultivars,we conducted a comparative analysis of anthocyanin metabolomics among white,green,and pink P.serrulata cultivars using LC-MS/MS.The results were as follows:(1)A total of 42 anthocyanins were detected,mainly including petunidin,delphinidin,flavonoids,malvidin,peonidin,cyanidin,pelargonidin,and procyanidins.(2)A total of 25 anthocyanins were identified as differential metabolites,including 11 down-regulated and 14 up-regulated ones,in which seven anthocyanins with higher abundance in the pink flower.(3)KEGG pathway annotation showed that the differential metabolites were significantly enriched in the anthocyanin biosynthesis pathway;the results of clustering showed that petunidin-3-O-glucoside was the key metabolite for flower color differences of P.serrulata cultivars.This study provides important insights into the metabolic mechanism of flower color differences in P.serrulata and a reference for novel color cultivars and variety breeding.

关 键 词：山樱花 花色 代谢组 花青素 代谢通路 

分 类 号：Q943[生物学—植物学]