利用纤维素酶水解碱处理稻草生产葡萄糖  

Production of Glucose Using Cellulase Hydrolyzed Alkali Treatment for Rice Straw

作　　者：顾天华 曹亚群 李烜琦 任晓冬[1] GU Tianhua;CAO Yaqun;LI Xuanqi;REN Xiaodong(School of Life Sciences,Jilin University,Changchun 130012,China;Inner Mongolia Autonomous Region Urban and Rural Human Settlement Environment Development Promotion Center,Hohhot 010020,China)

机构地区：[1]吉林大学生命科学学院,长春130012 [2]内蒙古自治区城乡人居环境发展促进中心,呼和浩特010020

出　　处：《吉林大学学报（理学版）》2023年第3期702-706,共5页Journal of Jilin University:Science Edition

基　　金：内蒙古自治区科技计划项目(批准号:2019GG032).

摘　　要：针对木质纤维素结构复杂、难以降解利用,焚烧时导致环境污染的问题,利用纤维素酶将木质纤维素中的纤维素水解为葡萄糖.首先,使用NaOH溶液对稻草进行预处理;其次,对预处理前后的稻草进行扫描电镜、Fourier变换红外光谱和X射线衍射分析;最后,使用Aspergillus niger Q7生产的纤维素酶水解预处理稻草.结果表明:预处理后的稻草纤维结构被打开,表面积增大,结晶度上升;预处理稻草纤维素含量上升,半纤维素和木质素含量下降;预处理后比预处理前的稻草产生的葡萄糖量高.Aming at the problem that the lignocellulose had a complex structure and was difficult to degrade and utilize,resulting in environmental pollution when it burned,we used cellulase to hydrolyze cellulose from lignocellulose into glucose.Firstly,the rice straw was pretreated using sodium hydroxide solution.Secondly,the rice straw before and after the pretreatment was analyzed by scanning electron microscopy,Fourier transform infrared spectroscopy,and X-ray diffraction.Finally,the cellulase produced by Aspergillus niger Q7 was used to hydrolyze and pretreat rice straw.The results show that after pretreatment,the fiber structure of rice straw is opened,the surface area increases,and the crystallinity increases.The cellulose content in rice straw increases after pretreatment,while the content of hemicellulose and lignin decreases.The glucose content of pretreated rice straw is higher than that of unpretreated rice straw.

关 键 词：稻草 预处理 纤维素 纤维素酶 酶解 

分 类 号：Q93[生物学—微生物学]