高效液相色谱法测定复合营养素补充剂中叶酸含量  

Determination of Folic Acid in Complex Nutrients Supplementation by High Performance Liquid Chromatography

作　　者：陆伟 王俊 靳利军 熊菲菲 晏永球 贾福怀 Lu Wei;Wang Jun;Jin Lijun;Xiong Feifei;Yan Yongqiu;Jia Fuhuai(Ningbo Yufangtang Biological Technology Co.,Ltd.,Ningbo 315012,China;Yufangtang Natural Health Products Key Agricultural Enterprise Research Institute,Ningbo 315012,China;Ningbo Yuyi Biological Technology Co.,Ltd.,Ningbo 315012,China)

机构地区：[1]宁波御坊堂生物科技有限公司,浙江宁波315012 [2]御坊堂天然健康产品重点农业企业研究院,浙江宁波315012 [3]宁波御益生物科技有限公司,浙江宁波315012

出　　处：《山东化工》2023年第4期127-130,共4页Shandong Chemical Industry

摘　　要：目的:通过建立一种提取效率高、耐复杂基质干扰能力强的样品前处理方式结合高效液相色谱紫外(High performance liquid chromatography-ultraviolet,HPLC-UV)检测方法,解决复合营养素补充剂中叶酸的准确定量检测难题。方法:以多种维生素矿物质片为特征样品,首次采用弱碱性饱和乙二胺四乙酸二钠盐(EDTA)溶液为样品提取液并结合操作简便的超声提取前处理方法,高效提取供试品中的叶酸成分并有效屏蔽基质因素干扰,最终使用HPLC分离高灵敏检测。结果:叶酸在浓度范围0.9912~49.5615μg/mL内保持良好现行关系(r2=0.9999);加标回收率达99.08%;平行试验结果RSD=1.01%(n=6);方法定量限LOQ=0.15μg/mL。改进后的分析方法通过系统的方法学验证后发现能够针对多种维生素矿物质片中的叶酸进行准确定量,并成功应用于市面上多种剂型叶酸类保健食品的实际检测中,结果均与产品标示量保持一致。结论:本方法简便易操作、提取效率高、抗基质干扰能力强、应用范围广,适用于多种维生素矿物质片中的叶酸含量测定,并为复合营养素补充剂类保健食品中叶酸测检测提供了一条新的途径。Objective:To establish a high effect and strong anti-jamming ability analysis method for determination of folic acid in complex nutrients supplementation by high performance liquid chromatography-ultraviolet(HPLC-UV).Methods:Using vitamins and minerals tablets as the representative sample,folic acid was extracted by alkalescent saturated ethylenediamine tetraacetic acid disodium salt(EDTA)extraction solution for the first time with ultrasonic extraction pretreatment method,and it was also protected well at the same time.The extracting solution was detected by HPLC.Results:The assay was validated in the concentration range of 0.9912~49.5615μg/mL(r2=0.9999).The average recovery was 99.08%.The results of parallel experiments RSD=1.01%(n=6).Method limit of quantification LOQ=0.15μg/mL.After the systematic validation,the improved method has been successfully applied to detect folic acid in compound vitamins and minerals tablets and complex matrix functional food.Conclusion:The new method is easy to operate,with high extraction efficiency and strong anti-jamming ability,and can be used for determination of folic acid in compound vitamins and minerals tablets and many other complex matrix functional food.It also provides a new approach for determination of folic acid in functional food-complex nutrients supplementation.

关 键 词：叶酸 多种维生素矿物质片 复合营养素补充剂 保健食品 HPLC-UV 

分 类 号：TQ460[化学工程—制药化工] R927[医药卫生—药学]