贝类毒素大田软海绵酸的免疫分析检测技术研究进展  

Recent progress in immunoassay detection technology of shellfish-toxin okadaic acid

作　　者：王峥 李佳钰 匡佳妮 赵辰昊 张智 任振俶 秦源 WANG Zheng;LI Jia-Yu;KUANG Jia-Ni;ZHAO Chen-Hao;ZHANG Zhi;REN Zhen-Chu;QIN Yuan(College of Life Sciences and Medicine,Zhejiang Sci-tech University,Hangzhou 310018,China;Zhejiang Sci-tech University Shaoxing Academy of Biomedicine Co.,Ltd.,Shaoxing 312000,China;Lingbo Biotechnology(Hangzhou)Co.,Ltd.,Hangzhou 310018,China)

机构地区：[1]浙江理工大学生命科学与医药学院,杭州310018 [2]浙江理工大学绍兴生物医药研究院有限公司,绍兴312000 [3]领博生物科技(杭州)有限公司,杭州310018

出　　处：《食品安全质量检测学报》2023年第6期100-107,共8页Journal of Food Safety and Quality

基　　金：浙江省自然科学基金项目(LQ23H050005);浙江理工大学绍兴生物医药研究院有限公司开放课题基金项目(SXAB202207);国家级大学生创新创业训练计划基金项目(202210338050)。

摘　　要：大田软海绵酸(okadaicacid,OA)是一种广泛存在于贝类等生物中的海洋生物毒素,可引起人或动物的急性中毒,对食品安全和海产养殖具有严重危害。因此建立快速、可靠、灵敏的OA检测技术具有重要意义。免疫分析检测技术基于抗原抗体的结合,特异性强、灵敏度高、应用范围广,是当前检测贝类毒素OA的主要手段。本文综述了近年来针对贝类毒素OA的免疫分析检测技术,其中包括酶联免疫吸附检测、免疫层析检测、时间分辨荧光免疫检测和基于免疫传感器的检测技术等。本文着重阐述了不同免疫分析技术的原理及其在OA检测中的实际应用,同时探讨了免疫分析技术在贝类毒素OA检测方面的挑战和发展趋势,以期为开发性能更加优异的OA免疫检测技术提供研究思路。Okadaic acid(OA)is a marine biotoxin widely found in shellfish,which can cause acute poisoning in humans or animals,and has serious harm to food safety and marine aquaculture.Therefore,it is of great significance to establish a fast,reliable,and sensitive OA detection technology.Immunoassay detection technology is based on the combination of antigen and antibody,with strong specificity,high sensitivity,and wide application range,which is currently the main method for shellfish-toxin OA detection.This work reviewed the immunoassay techniques for shellfish-toxin OA in recent years,including enzyme-linked immunosorbent assay,immunochromatographic assay,time resolved fluoroimmunoassay,and immunosensor based detection techniques.This paper focused on the principles of different immunoassay techniques and their practical applications in OA detection,and discussed the development trends and challenges of immunoassay techniques in the detection of shellfish-toxin OA,in order to provide more new ideas to the authors for developing OA immunoassay techniques with better performance.

关 键 词：生物毒素 腹泻性贝类毒素 大田软海绵酸 免疫分析检测 

分 类 号：TS254.7[轻工技术与工程—水产品加工及贮藏工程]