The immunoregulatory role of fish specific type Ⅱ SOCS via inhibiting metaflammation in the gut-liver axis  

作　　者：Qingsong Zhu Yuhang Liu Yingyin Cheng Wanting Zhang Nan Wu Xiao-Qin Xia 

机构地区：[1]College of Fisheries and Life Science,Dalian Ocean University,Dalian,China [2]Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan,China [3]College of Advanced Agricultural Sciences,University of Chinese Academy of Sciences,Beijing,China

出　　处：《Water Biology and Security》2023年第2期49-62,共14页水生生物与安全（英文）

基　　金：funded by the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA24010206);the National Natural Science Fundation of China(31872592).

摘　　要：SOCS8,also known as CISHb,is a fish-specific type Ⅱ SOCS.Because CISH binds to cytokine receptors and may inhibit STAT5 activation(a substrate of the insulin receptor),SOCS8 may be involved in the control of metaflammation.The socs8-/-zebrafish were created,and both longer trunks and intestinal villi were observed in 1-month-old(mo)fish.Altered mucosal immunity and gut-liver metabolism were also found in socs8-/-fish.Increased intestinal neutrophils and macrophages,together with overexpression of cytokines and T cell markers in this mutant fish,suggested SOCS8's immunoregulating role.During modeling of soybean-induced enteritis using the 3 mo zebrafish,lower expression levels of inflammatory genes but more mucosa barrier disruption were discovered in socs8-/-zebrafish,compared with wide type counterparts.Furthermore,the shrunk villi at 6 mo in socs8-/-fish suggested that the mucosa might have been protected by SOCS8.This is also consistent with the assertion that metaflammation eventually leads to tissue degeneration and premature death.The fact that socs8-/-fish had more hepatic oil droplets compared to their wild-type counterparts suggested SOCS8's role in inhibiting hepatic metaflammation.Transcriptomic analysis as well as 16S rRNA gene sequencing were done on 3 mo socs8-/-fish to methodically reveal the altered immunity and metabolic reprogramming in the gut and liver caused by socs8-/-.The enriched GO terms for the intestinal tract,such as"cytokine-mediated signaling pathway"and"response to external biotic stimulus",as well as KEGG pathways in both gut and liver like"carbon metabolism"and"glycolysis/gluconeogenesis",were consistent with previously revealed pathological clues and improved growth performance at early age,respectively.In addition,the microbiota in the socs8-/-strain had adapted to the host's increased carbohydrate metabolism,as evidenced by higher levels of Bacteroidota.Furthermore,Verrucomicrobiota associated with immunoregulation were found in lower abundance in socs8-/-fish.As a result,cur

关 键 词：Gut-liver axis IMMUNOREGULATION Insulin signaling SOCS8 ZEBRAFISH 

分 类 号：S94[农业科学—水产养殖]