Major royal-jelly proteins intake modulates immune functions and gut microbiota in mice  

作　　者：Hang Wu Shican Zhou Wenjuan Ning Xiao Wu Xiaoxiao Xu Zejin Liu Wenhua Liu Kun Liu Lirong Shen Junpeng Wang 

机构地区：[1]Infection and Immunity Institute and Translational Medical Center of Huaihe Hospital,Henan University,Kaifeng 475000,China [2]College of Biology Science and Engineering,Hebei University of Economics and Business,Shijiazhuang 050061,China [3]College of Biosystems Engineering and Food Science,Zhejiang Key Laboratory for Agro-Food Processing,Zhejiang University,Hangzhou 310058,China

出　　处：《Food Science and Human Wellness》2024年第1期444-453,共10页食品科学与人类健康（英文）

基　　金：financially supported by the National Natural Science Foundation of China(U2004104);the Natural Science Foundation of Henan Province(202300410080);the Key Project of Henan Education Committee(21A310005);the Internal Fund of Hebei University of Economics and Business(2020ZD10);the Postgraduate“Talent Program”of Henan University(SYL20060187 and SYL20060189)。

摘　　要：In this study,we investigated the effects of major royal jelly proteins(MRJPs)on the estrogen,gut microbiota,and immunological responses in mice.Mice given 250 or 500 mg/kg,not 125 mg/kg of MRJPs,enhanced the proliferation of splenocytes in response to mitogens.The splenocytes and mesenteric lymphocytes activated by T-cell mitogens(Con A and anti-CD3/CD28 antibodies)released high levels of IL-2 but low levels of IFN-γand IL-17A.The release of IL-4 was unaffected by MRJPs.Additionally,splenocytes and mesenteric lymphocytes activated by LPS were prevented by MRJPs at the same dose as that required for producing IL-1βand IL-6,two pro-inflammatory cytokines.The production of IL-1β,IL-6,and IFN-γwas negatively associated with estrogen levels,which were higher in the MRJP-treated animals than in the control group.Analysis of the gut microbiota revealed that feeding mice 250 mg/kg of MRJPs maintained the stability of the natural intestinal microflora of mice.Additionally,the LEf Se analysis identified biomarkers in the MRJP-treated mice,including Prevotella,Bacillales,Enterobacteriales,Gammaproteobacteria,Candidatus_Arthromitus,and Shigella.Our results showed that MRJPs are important components of royal jelly that modulate host immunity and hormone levels and help maintain gut microbiota stability.

关 键 词：Major royal-jelly proteins Immunity ESTROGEN Gut microbiota Cytokines 

分 类 号：TS201.4[轻工技术与工程—食品科学]