Single-cell-resolution transcriptome map revealed novel genes involved in testicular germ cell progression and somatic cells specification in Chinese tongue sole with sex reversal  

作　　者：Hong-Yan Wang Xiang Liu Jian-Yang Chen Yingyi Huang Yifang Lu Fujian Tan Qun Liu Mingming Yang Shuo Li Xianghui Zhang Yating Qin Wenxiu Ma Yingming Yang Liang Meng Kaiqiang Liu Qian Wang Guangyi Fan Rafael H.Nóbrega Shanshan Liu Francesc Piferrer Changwei Shao 

机构地区：[1]Key Lab of Sustainable Development of Marine Fisheries,Ministry of Agriculture and Rural Affairs,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266072,China [2]Laboratory for Marine Fisheries Science and Food Production Processes,Qingdao National Laboratory for Marine Science and Technology,Qingdao 266071,China [3]College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China [4]BGI Research-Qingdao,BGI,Qingdao 266555,China [5]BGI-Shenzhen,Shenzhen 518083,China [6]School of Marine Sciences,Ningbo University,Ningbo 315211,China [7]College of Marine Technology and Environment,Dalian Ocean University,Dalian 116023,China [8]Qingdao-Europe Advanced Institute for Life Sciences,BGI-Shenzhen,Qingdao 266555,China [9]College of Life Sciences,Shandong Normal University,Jinan 250014,China [10]Reproductive and Molecular Biology Group,Department of Structural and Functional Biology,Institute of Biosciences,São Paulo State University(UNESP),Botucatu,São Paulo,Brazil [11]Institut de Ciències del Mar(ICM),Spanish National Research Council(CSIC),Barcelona,Spain

出　　处：《Science China(Life Sciences)》2023年第5期1151-1169,共19页中国科学（生命科学英文版）

基　　金：supported by the National Key R&D Program of China(2018YFD0900301);the National Nature Science Foundation of China(31722058,31802275,31472269);the AoShan Talents Cultivation Program Supported by Qingdao National Laboratory for Marine Science and Technology(2017ASTCP-ES06);the Taishan Scholar Project Fund of Shandong of China to C.S.;the National Ten-Thousands Talents Special Support Program to C.S.;the Central Public-interest Scientific Institution Basal Research Fund,CAFS(2020TD19);the China Agriculture Research System(CARS-47-G03)。

摘　　要：Female-to-male sex reversals(pseudomales)are common in lower vertebrates and have been found in natural populations,which is a concern under rapid changes in environmental conditions.Pseudomales can exhibit altered spermatogenesis.However,the regulatory mechanisms underlying pseudomale spermatogenesis remain unclear.Here,we characterized spermatogenesis in Chinese tongue sole(Cynoglossus semilaevis),a species with genetic and environmental sex determination,based on a highresolution single-cell RNA-seq atlas of cells derived from the testes of genotypic males and pseudomales.We identified five germ cell types and six somatic cell types and obtained a single-cell atlas of dynamic changes in gene expression during spermatogenesis in Chinese tongue sole,including alterations in pseudomales.We detected decreased levels of Ca^(2+)signaling pathway-related genes in spermatogonia,insufficient meiotic initiation in spermatocytes,and a malfunction of somatic niche cells in pseudomales.However,a cluster of CaSR genes and MAPK signaling factors were upregulated in undifferentiated spermatogonia of pseudomales.Additionally,we revealed that Z chromosome-specific genes,such as piwil2,dhx37,and ehmt1,were important for spermatogenesis.These results improve our understanding of reproduction after female-to-male sex-reversal and provide new insights into the adaptability of reproductive strategies in lower vertebrates.

关 键 词：Cynoglossus semilaevis SPERMATOGENESIS single-cell transcriptome sex reversal SPERMATOGONIA 

分 类 号：Q492[生物学—生理学]