全自动免疫磁珠法与免疫亲和柱法测定食用油中黄曲霉毒素B1的对比研究  

Comparative Study on the Determination of Aflatoxin B1 in Edible Oil by Automated Immunomagnetic Beadpurification Method and Immunoaffinity Column Purification Method

作　　者：曹晶晶 刘莹 王雅楠 赵胜男 毋修远 高海军 CAO Jing-jing;LIU Ying;WANG Ya-nan;ZHAO Sheng-nan;WU Xiu-yuan;GAO Hai-jun(Henan Insitute of Food and Salt Industry Inspection Technology,Zhengzhou,Henan 450003,China)

机构地区：[1]河南省食品和盐业检验技术研究院,河南郑州450003

出　　处：《粮油食品科技》2023年第3期153-158,共6页Science and Technology of Cereals,Oils and Foods

摘　　要：采用全自动免疫磁珠净化与免疫亲和柱净化的前处理方法,结合超高效液相色谱法对不同食用油中黄曲霉毒素B1进行定量检测,并对两种前处理方法结果准确性、加标回收率、检测时间进行了对比。结果表明:全自动免疫磁珠净化与免疫亲和柱净化测定植物油标准物质,结果分别为15.59μg/kg和14.64μg/kg,均在标准值范围内;对不同种类食用油添加不同量的黄曲霉毒素B1标准物质,加标回收率均在88%~110%之间,相对标准偏差均在5%以内。采用Bland-Altman法对全自动免疫磁珠净化法和免疫亲和柱净化法进行差异分析,结果显示这两种净化方法的差异在可接受范围内。两种方法在净化和富集食用油中黄曲霉毒素B1均可满足实验要求,可以互相代替使用。通过检测时间对比,全自动免疫磁珠净化搭配使用真菌毒素全自动净化仪前处理方法可同时处理多个样品,平均一个样品净化时间小于3 min,处理时间短、实验效率更高。In this experiment the pretreatment methods of automatic immunomagnetic bead purification and immunoaffinity chromatography purification were used to quantitatively detect aflatoxin B1 in different edible oils by ultra-high performance liquid chromatography,and the accuracy,spike recovery and detection time of the two pretreatment methods were compared.The results showed that the results of automatic immunomagnetic bead purification and immunoaffinity chromatography purification for the determination of vegetable oil standard materials were 15.59μg/kg and 14.64μg/kg,respectively,both of which were within the standard range.Different amounts of aflatoxin B1 standard materials were added to different kinds of edible oils,and the recovery rate of spikes was between 88%~110%,and the relative standard deviation was within 5%.The Bland-Altman method was used to analyze differences between the automatic immunomagnetic bead purification method and the immunoaffinity chromatography purification method,and the results showed that differences between the two purification methods were within the acceptable range.Both methods could meet the experimental requirements in purification and enrichment of aflatoxin B1 in edible oil and can be used instead of each other.Through the comparison of detection time,automatic immunomagnetic bead purification combined with mycotoxin automatic purification instrument pretreatment method can process multiple samples at the same time,and the average purification time of one sample is less than 3 min,indicating that the treatment time is shorter,and the experimental efficiency is higher.

关 键 词：全自动免疫磁珠 免疫亲和柱 超高效液相色谱法 黄曲霉毒素B1 食用油 

分 类 号：TS207.7[轻工技术与工程—食品科学] S-3[轻工技术与工程—食品科学与工程]