肉与肉制品中食源性致病微生物快速检测技术研究进展  

Research progress on rapid detection technology of foodborne pathogenic microorganisms in meat and meat products

作　　者：孙新城 李侠颖 许素月 李爽 周杰 白艳红 SUN Xin-Cheng;LI Xia-Ying;XU Su-Yue;LI Shuang;ZHOU Jie;BAI Yan-Hong(College of Food and Bioengineering,Zhengzhou University of Light Industry,Zhengzhou 450001,China;Henan Provincial Key Laboratory of Cold Chain Food Quality Safety and Control,Zhengzhou 450001,China;Food Production and Safety Henan Collaborative Innovation Center,Zhengzhou 450001,China)

机构地区：[1]郑州轻工业大学食品与生物工程学院,郑州450001 [2]河南省冷链食品质量安全与控制重点实验室,郑州450001 [3]食品生产与安全河南省协同创新中心,郑州450001

出　　处：《食品安全质量检测学报》2023年第7期32-38,共7页Journal of Food Safety and Quality

基　　金：河南省重大公益项目(201300110100);河南省重点研发项目(222102520041);河南省科技研发联合基金项目(222103810020);河南省人社厅留学回国人员择优支持项目(2020039);郑州市协同创新专项(2021ZDPY0202)。

摘　　要：随着中国公民的饮食结构不断变化,肉与肉制品在日常生活中的比例不断增加,肉与肉制品已成为食源性疾病和公共卫生问题的重要来源。针对肉与肉制品基质复杂、致病微生物浓度低的特点,传统的食源性微生物检测方法耗时长、操作过程复杂,已不能满足现代食品检测的要求,以分子生物学、免疫分析、生物传感器、核酸适配体为基础的快速检测方法发展迅速,已经成为食源性致病微生物检测的主要方法。本文主要从分子生物学、免疫分析、生物传感器、核酸适配体检测技术等出发综述肉与肉制品中食源性致病微生物的检测方法,并总结各检测技术的优缺点,为开辟肉与肉制品中食源性致病微生物检测新方法提供参考。With the changing diet of Chinese citizens and the increasing proportion of meat and meat products in daily life,meat and meat products have become an important source of foodborne illness and public health problems.In view of the complex matrix of meat and meat products and the low concentration of pathogenic microorganisms,the traditional detection methods of foodborne microorganisms are time-consuming and complicated,which cannot meet the requirements of modern food detection.The rapid detection methods based on molecular biology,immunoassay,biosensor and nucleic acid aptamer have developed rapidly and have become the main methods for the detection of foodborne pathogenic microorganisms.This paper mainly reviewed the detection methods of foodborne pathogenic microorganisms in meat and meat products from the perspectives of molecular biology,immunoassay,biosensor and nucleic acid aptamer detection technology,etc.,and summarized the advantages and disadvantages of each detection technology,which provides references for opening up new detection methods of foodborne pathogenic microorganisms in meat and meat products.

关 键 词：食源性致病菌 快速检测 肉与肉制品 传感器 

分 类 号：TS251.7[轻工技术与工程—农产品加工及贮藏工程]