Assessment of thiol/disulfide homeostasis for prognostic follow-up of cholangiocarcinoma patients  

作　　者：Zozan Guleken Huri Bulut Kursat Rahmi Serin Melek Buyuk Engin Ulukaya 

机构地区：[1]Department of Physiology,Faculty of Medicine,Gaziantep Islam Science and Technology University,Kucukkızılhisar 27220,Gaziantep,Turkey [2]Department of Medical Biochemistry,Faculty of Medicine,Istinye University,Istanbul 34010,Turkey [3]Department of General Surgery Hepato-pancreato-biliary Surgery Unit,Faculty of Medicine,Istanbul University,Istanbul 34390,Turkey [4]Department of Pathology,Faculty of Medicine,Istanbul University,Istanbul 34390,Turkey [5]Molecular Cancer Research Center(ISUMKAM),Istinye University,Istanbul 34010,Turkey

出　　处：《Cancer Advances》2023年第9期1-8,共8页TMR肿瘤进展

摘　　要：Background:Cholangiocarcinoma(CCA)is a cancer type that builds in the bile ducts that carry digestive fluid,bile.These ducts connect the liver to the gallbladder and the small intestine.The disease is often diagnosed at an advanced stage,resulting in a low 5-year survival rate.This study aims to evaluate the concentrations of serum thiol/disulfide in CCA patients and healthy volunteers and investigate the association between oxidative activity and clinical and pathological characteristics in CCA patients.By examining the relationship between reduced thiol/disulfide measures and tumorigenesis of the disease,we can potentially identify an unfavourable prognosis in CCA patients.Methods:To assess the status of thiol/disulfide in the blood of Cholangiocarcinoma patients using a novel automated homeostasis assay,we recruited 55 individuals for this study(CCA patients,n=27;healthy volunteers,n=28).We measured the levels of serum total thiol(TT)(–SH+–S-S–),native thiol(NT)(–SH),and disulfide(DD)(–S-S–)in both the CCA group and the control group.Additionally,we calculated the ratio of thiol/disulfide(–SH/–S-S–).Results:We explored the relationship between oxidative activity and clinical and pathological characteristics in CCA patients.The study found no significant differences in age and sex between CCA patients and controls.NT and TT levels were higher in the control group compared to the CCA group.However,the disulfide level did not significantly differ between the two groups.Pearson's correlation matrix analysis revealed positive correlations between TT and NT levels and negative correlations between NT/TT%,DD level,and DD/TT%.Conclusion:Our findings suggest a statistical difference in serum thiol/disulfide parameters.As a marker of total oxidant status,thiol and disulfide levels have decreased in patients with CCA.However,no correlation was found between the tumour markers CEA and CA19-9 and thiol levels.A decrease in blood thiol and disulfide concentrations or the ratio of thiol to disulfide may ind

关 键 词：CHOLANGIOCARCINOMA native thiol total thiol dynamic disulphide oxidative stress 

分 类 号：R73[医药卫生—肿瘤]