原儿茶酸通过Toll样受体4/核因子κB途径减轻高脂饮食诱导的肝脏炎症  

Intake of Protocatechuic Acid Attenuates High-Fat Diet Induced Liver Inflammation via the Toll Like Receptor 4/Nuclear FactorκB Pathway

作　　者：焦小文 李佳 李云龙 韩林 王敏[1] JIAO Xiaowen;LI Jia;LI Yunlong;HAN Lin;WANG Min(College of Food Science and Engineering,Northwest A&F University,Yangling 712100,China;Institute of Functional Food of Shanxi,Shanxi Agricultural University,Taiyuan 030031,China)

机构地区：[1]西北农林科技大学食品科学与工程学院,陕西杨凌712100 [2]山西农业大学山西功能食品研究所,山西太原030031

出　　处：《食品科学》2023年第9期72-81,共10页Food Science

基　　金：国家自然科学基金青年科学基金项目(32001701);国家现代农业产业技术体系建设专项(CARS-08-E2-1)。

摘　　要：原儿茶酸(protocatechuic acid,PCA)是花青素在体内的主要代谢产物之一,具有良好的抗炎活性。本实验利用高脂饮食(high fat diet,HFD)诱导C57BL/6J小鼠肝脏炎症,通过灌胃100 mg/(kg mb·d)PCA研究其对小鼠肝脏的保护效果,并通过体外实验进一步研究其潜在机制。体内研究结果表明,PCA干预12周后,可显著降低HFD诱导C57BL/6J小鼠的体质量和肝脏脂肪含量;血清和肝脏中白细胞介素(interleukin,IL)-1β、IL-6、肿瘤坏死因子α(tumor necrosis factorα,TNF-α)和脂多糖(lipopolysaccharide,LPS)等炎症因子水平均明显降低。体外转录组测序及原代肝细胞和肝脏的免疫印迹分析结果表明,PCA可显著降低Toll样受体4(Toll like receptor 4,TLR4)基因和蛋白的表达,然后通过下调核因子κB(nuclear factorκB,NF-κB)的磷酸化抑制炎症因子(如IL-6)的表达。综上,PCA可有效改善HFD诱导的肝脏炎症,其可能是通过下调TLR4/NF-κB信号通路来实现。Protocatechuic acid(PCA)is one of the main metabolites of anthocyanins in the body and has good antiinflammatory activity.In this study,the protective effect of oral administration of PCA at a dose of 100 mg/(kg mb·d)on high-fat diet(HFD)-induced liver inflammation in C57BL/6J mice was investigated,and the underlying mechanism was investigated by in vitro experiments.In vivo studies showed that after 12 weeks of PCA intervention,the body mass and liver fat content of HFD-fed C57BL/6J mice were significantly reduced,and so were the levels of inflammatory factors such as interleukin(IL)-1β,IL-6,tumor necrosis factorα(TNF-α)and lipopolysaccharide(LPS)in serum and liver.In vitro transcriptome sequencing(RNA-Seq)and Western blot analysis of primary hepatocytes and livers demonstrated that PCA could significantly reduce the gene and protein expression of Toll like receptor 4(TLR4),and then down-regulate the phosphorylation of nuclear transcription factorκB(NF-κB)inhibiting the expression of inflammatory factors such as IL-6.These results suggest that PCA can effectively improve HFD-induced liver inflammation,possibly by down-regulating the TLR4/NF-κB signaling pathway.

关 键 词：原儿茶酸 肝脏炎症 转录组测序 TOLL样受体4 核因子ΚB 

分 类 号：TS201.4[轻工技术与工程—食品科学]