Molecular toxicity and defense mechanisms induced by silver nanoparticles in Drosophila melanogaster  

作　　者：Zhidi Wang Liying Zhang Xing Wang 

机构地区：[1]College of Resources and Environmental Sciences,China Agricultural University,Beijing 100193,China [2]Beijing Key Laboratory of Biodiversity and Organic Farming,Beijing 100193,China

出　　处：《Journal of Environmental Sciences》2023年第3期616-629,共14页环境科学学报（英文版）

基　　金：supported by the Natural Science Foundation of Beijing, China (No. 5212012);the National Natural Science Foundation of China (No. 31801190);the 2115 Talent Development Program of China Agricultural University。

摘　　要：The widely use of silver nanoparticles(AgNPs) as antimicrobial agents gives rise to potential environmental risks. AgNPs exposure have been reported to cause toxicity in animals.Nevertheless, the known mechanisms of AgNPs toxicity are still limited. In this study, we systematically investigated the toxicity of AgNPs exposure using Drosophila melanogaster.We show here that AgNPs significantly decreased Drosophila fecundity, the third-instar larvae weight and rates of pupation and eclosion in a dose-dependent manner. AgNPs reduced fat body cell viability in MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)assays. AgNPs caused DNA damage in hemocytes and S2 cells. Interestingly, the mRNA levels of the entire metallothionein gene family were increased under AgNPs exposure as determined by RNA-seq analysis and validated by qRT-PCR, indicating that Drosophila responded to the metal toxicity of AgNPs by producing metallothioneins for detoxification. These findings provide a better understanding of the mechanisms of AgNPs toxicity and may provide clues to effect on other organisms, including humans.

关 键 词：Drosophila melanogaster Silver nanoparticles(AgNPs) Reactive oxygen species(ROS) DNA damage RNA-SEQ METALLOTHIONEIN 

分 类 号：X503[环境科学与工程—环境工程]