Developmental toxicity of TCBPA on the nervous and cardiovascular systems of zebrafish (Danio rerio): A combination of transcriptomic and metabolomics  

作　　者：Wentao Liu Yifan Pan Lu Yang Yun Xie Xuanyue Chen Jing Chang Weiyu Hao Lifei Zhu Bin Wan 

机构地区：[1]Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China [2]University of Chinese Academy of Sciences,Beijing 100049,China [3]Agricultural Information Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China [4]Institute of Food Safety,Chinese Academy of Inspection and Quarantine,Beijing 100176,China [5]Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China

出　　处：《Journal of Environmental Sciences》2023年第5期197-209,共13页环境科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos.22076214 and 42007226);Beijing Municipal Youth Top-Notch Talent Program(No.2018000021223ZK34).

摘　　要：Tetrachlorobisphenol A(TCBPA),a widely used halogenated flame retardant,is frequently detected in environmental compartments and human samples.However,unknown developmental toxicity and mechanisms limit the entire understanding of its effects.In this study,zebrafish(Danio rerio)embryos were exposed to various concentrations of TCBPA while a combination of transcriptomics,behavioral and biochemical analyzes as well as metabolomics were applied to decipher its toxic effects and the potential mechanisms.We found that TCBPA could interfere with nervous and cardiovascular development through focal adhesion and extracellular matrix-receptor(ECM-receptor)interaction pathways through transcriptomic analysis.Behavioral and biochemical analysis results indicated abnormal swimming behavior of zebrafish larvae.Morphological observations revealed that TCBPA could cause the loss of head blood vessels.Metabolomic analysis showed that arginine-related metabolic pathways were one of the main pathways leading to TCBPA developmental toxicity.Our study demonstrated that by using omics,TCBPA was shown to have neurological and cardiovascular developmental toxicity and the underlying mechanisms were uncovered and major pathways identified.

关 键 词：Tetrachlorobisphenol A(TCBPA) ZEBRAFISH Developmental toxicity TRANSCRIPTOMICS Metabolomics 

分 类 号：X171.5[环境科学与工程—环境科学]