灭草松对葛仙米的生理毒害效应  

THE PHYSIOLOGICAL TOXIC EFFECTS OF BENTAZONE ON NOSTOC SPHAEROIDS KÜTZING

作　　者：刘树文 许俊党 刘欢 聂嘉音 李宝蕾 李玉仙 LIU Shu-Wen;XU Jun-Dang;LIU Huan;NIE Jia-Yin;LI Bao-Lei;LI Yu-Xian(School of Biological Sciences,Guizhou Normal College,Guiyang 550018,China)

机构地区：[1]贵州师范学院生物科学学院,贵阳550018

出　　处：《水生生物学报》2023年第7期1060-1068,共9页Acta Hydrobiologica Sinica

基　　金：国家自然科学基金(31660115);贵州省科技基金(黔科合J字[2013]2234号)资助。

摘　　要：为研究灭草松对葛仙米(Nostoc sphaeroids Kützing)的生理毒害效应,文章比较分析了不同时间和不同浓度灭草松处理对葛仙米的生长、氧化与抗氧化系统及光合与呼吸等方面的生理毒害效应。结果表明,灭草松抑制了葛仙米的生长,葛仙米细胞内超氧阴离子(O_(2)^(-))产生速率和双氧水(H_(2)O_(2))、还原型抗坏血酸(AsA)、丙二醛(MDA)、脯氨酸(Pro)含量及过氧化氢酶(CAT)、过氧化物酶(POD)两种抗氧化酶活性随着灭草松胁迫浓度的升高及时间的延长,呈上升趋势。还原型谷胱甘肽(GSH)含量随着灭草松浓度的升高而先降低后升高,整体变化的幅度较小。超氧化物歧化酶(SOD)活性整体随着灭草松处理浓度的升高和时间的延长呈升高趋势,但高浓度的灭草松处理96h后SOD酶活性略小于48h。在灭草松处理葛仙米72h后,总产氧量和净产氧量随着灭草松处理浓度的升高而不同程度的降低,但呼吸耗氧量无显著差异,高浓度灭草松处理葛仙米的总产氧量更容易受到强光抑制且弱光恢复能力降低。研究结果为进一步研究葛仙米对灭草松的毒害与适应的分子机制及野生葛仙米的生态保护提供参考。Ge-Xian-Mi(Nostoc sphaeroids Kützing)grown in paddy fields is a rare edible cyanobacteria.The yield of wild resources of Ge-Xian-Mi has decreased owing to the extensive use of pesticides and fertilizers.The physiological toxic effects on Ge-Xian-Mi was lack of studies for bentazone,although it was a broad-spectrum and high-efficiency and low-toxicity new herbicide.This study compared and analyzed the physiological toxic effects in growth,oxidation and antioxidant system,photosynthesis and respiration of Ge-Xian-Mi treated by different times and different concentrations of bentazone.The results showed that bentazone inhibited the growth of Ge-Xian-Mi.In the wake of increased concentration and prolonged treatments for bentazone,they showed an upward trend as production rate of and the content of H_(2)O_(2),AsA,MDA,Pro and the activities of two antioxidant enzymes,CAT and POD in Ge-Xian-Mi.With the increased concentrations of bentazone,the content of GSH in Ge-Xian-Mi firstly decreased and then increased,but it was little changed overall.The SOD enzyme activity increased with concentrations and time treatments for bentazone,but the SOD enzyme activity was slightly less than 48h after treatment with high concentration of bentazone for 96h.After treated with bentazone for 72h,the total oxygen production and net oxygen production of Ge-Xian-Mi decreased with the increased concentrations of bentazone,however,no significant difference was found in respiratory oxygen consumption.The total oxygen production in the treatment of Ge-Xian-Mi was more easily inhibited by strong light and the recovery ability of weak light was reduced.The results of this study provide a reference for further research on the molecular mechanism of the toxicity and adaptation of Ge-Xian-Mi to bentazone and the ecological protection of wild Ge-Xian-Mi.

关 键 词：生长抑制 生理毒害效应 除草剂 抗氧化 活性氧 灭草松 葛仙米 

分 类 号：Q945[生物学—植物学] X171.5[环境科学与工程—环境科学]