金属离子促进樟芝深层发酵无性产孢  

Metal ions promoting sporulation of Antrodia cinnamomea in submerged fermentation

作　　者：李华祥 王娟娟 石瑀 吉丹 高亚军 贾禄强 饶胜其[1] 杨振泉[1] LI Huaxiang;WANG Juanjuan;SHI Yu;JI Dan;GAO Yajun;JIA Luqiang;RAO Shengqi;YANG Zhenquan(School of Food Science and Engineering,Yangzhou University,Yangzhou 225127,China)

机构地区：[1]扬州大学食品科学与工程学院,江苏扬州225127

出　　处：《食品与发酵工业》2023年第10期55-63,共9页Food and Fermentation Industries

基　　金：国家自然科学基金(32001661);江苏省自然科学基金(BK20190890)。

摘　　要：樟芝(Antrodia cinnamomea)是一种珍稀药食两用蕈菌,其目前最主要的人工培养方式是基于无性孢子接种的深层发酵。为了提高樟芝深层发酵的产孢量及产孢速度,进而提高其种子制备效率及工业化生产效益,该文首先采用单因素法筛选出能显著促进樟芝无性产孢的金属离子,再采用响应面法优化金属离子组合的最佳添加浓度。结果表明,在培养基中单独添加1.0 mmol/L的Ca^(2+)、0.1 mmol/L的Fe^(2+)及0.1 mmol/L的Zn^(2+)可使樟芝最大产孢量较对照组分别提高87.21%、72.39%及35.05%;同时添加0.149 mmol/L的Fe^(2+)、2.01 mmol/L的Ca^(2+)及0.135 mmol/L的Zn^(2+)可使樟芝深层发酵最大产孢量达8.67×10^(7)个/mL,较对照组提高133.62%,同时使樟芝无性孢子产量达到最大值的时间缩短24 h。该研究操作方便、成本低廉、效果显著,具有较好的开发价值及应用前景。Antrodia cinnamomea is a precious and popular edible and medicinal mushroom.Submerged fermentation based on spore inoculation is considered the most efficient and popular artificial cultivation method to produce A.cinnamomea.Improving the yield and speed of sporulation is beneficial to improve the inoculum preparation efficiency and industrial production benefit of A.cinnamomea in submerged fermentation.Thus,the metal ions that can significantly promote sporulation of A.cinnamomea were selected by single factor method,then the response surface methodology was used to optimize the concentrations of the metal ion combination.It was found that separately adding 1.0,0.1,0.1 mmol/L of Ca^(2+),Fe^(2+),or Zn^(2+)to the medium markedly increased the sporulation of A.cinnamomea by 87.21%,72.39%,and 35.05%,respectively.Adding 0.149,2.02,0.135 mmol/L of Fe^(2+),Ca^(2+),and Zn^(2+)together,resulted in an 8.67×10^(7) spores/mL sporulation of A.cinnamomea,133.62%higher compared to that of the control.Meanwhile,the time for sporulation of A.cinnamomea reaching the maximum was shortened by 24 h.The present study is easy to operate,low-cost,and effective,thereby is of great value in development and application.

关 键 词：樟芝 深层发酵 无性产孢 金属离子 响应面 

分 类 号：S646[农业科学—蔬菜学]