Nano-Sustained CO-Releasing Molecules Alleviates Cyclosporin-A-Induced Nephrotoxicity and Renal Fibrosis by Inhibiting NLRP3 Inflammasome-Mediated TGF-β/Smad Signaling Pathway  

作　　者：Juan Ji Zhaoyu Bi Ling Tian Qian Zhang Shu-fen Hou Song Li 

机构地区：[1]Department of Nephrology,Affiliated Hospital of Hebei University,Baoding 071000,Hebei Province,China [2]Department of Urology,Affiliated Hospital of Hebei University,Baoding 071000,Hebei Province,China

出　　处：《Journal of Clinical and Nursing Research》2023年第3期149-156,共8页临床护理研究（英文）

基　　金：Health Commission of Hebei Province Chuanxiong:Extract Improves Inflammatory Response in Rats with Pyelonephritis through IL-6/STAT3 Signaling Pathway(Project number:20231486)。

摘　　要：Objective:To investigate the effect nano-sustained CO-releasing molecules on cyclosporin-A(CsA)-induced nephrotoxicity by inhibiting the NLRP3 inflammasome-mediated TGF-β/Smad signaling pathway.Methods:3×105 cell/mL human renal tubular epithelial cells(HK-2)and mouse primary cultured renal tubular epithelial cells(RTECs)were cultured under an inverted microscope and incubated with 10%DMEM and 0.25%β2M in NaCl solution for 3 h.HK-2 and RTECs were divided into 5 complex numbers.MTT assay was used to detect the relative proliferation level of one of the HK-2 cells and calculate the multiplication ratio.Results:The nano-sustained CO-releasing molecules CS-CO had a strong protective effect on the kidney.HK-2 and RTECs cells were treated with siRNA,inhibitors,and NLRP3 knockout mice,and the changes in cell activity and expression of intracellular inflammatory factors were studied.The expression of TGF-β1/Smad signaling pathway related proteins in HK-2 and RTECs was detected by ELISA,western blot,immunofluorescence,and other techniques.Conclusion:SMA/CORM2 alleviates CsA-induced renal fibrosis by inhibiting NLRP3 inflammasome-mediated TGF-β/Smad signaling pathway.

关 键 词：NLRP3 TGF-BETA Smad Renal fibrosis 

分 类 号：R73[医药卫生—肿瘤]