Optimization of Mycelial Culture Conditions and Fructification of Lentinus Species Using Rice Straw and Sawdust Based Substrates  

作　　者：Kalaw SP Dulay RMR Damaso EJ Ramos JC del Rosario MAG Abon MD De Leon AM Undan JR Reyes RG 

机构地区：[1]Tuklas Lunas Development Center,Central Luzon State University,Science City of Munoz,Nueva Ecija,Philippines 3120 [2]Department of Biological Sciences,College of Science,Central Luzon State University,Science City of Munoz,Nueva Ecija,Philippines 3120

出　　处：《Studies in Fungi》2021年第1期519-530,共12页真菌研究（英文）

基　　金：This work was funded by the Philippine Council for Health Research and Development, Department of Science and Technology。

摘　　要：Lentinus(Basidiomycota,Polyporaceae)species are naturally-occurring mushrooms with nutritional and pharmacological importance.Unlike shiitake Lentinula edodes,a widely cultivated mushroom,Lentinus species are often ignored because they are underutilized and unpopular.However,like shiitake,Lentinus spp.could also be valuable resources of functional food and bioactive compounds.It is therefore of high interest to understand their cultural requirements leading to their efficient biomass production.Thus,this study established the optimal culture conditions for the maximum mycelial growth of seven wild strains under four Lentinus species.Their cultivation potentials were also determined using rice straw and sawdust(7:3 v/v)substrate formulation.Mycelia of all Lentinus strains evaluated favorably grew on coconut water gulaman(local crude agar)and other culture media for specific strain with suitable pH ranging from 5.0 to 8.0.Aeration was not a major physical factor for all Lentinus strains except L.squarrosulus strain 1,which favored sealed condition.Both lighted and dark conditions were found suitable for both strains of L.sajor-caju,L.strigosus strain 2 and L.swartzii whereas both strains of L.squarrosulus and L.strigosus strain 1 favorably grew in lighted condition.However,all Lentinus strains evaluated recorded the highest mycelial growth rates and thick mycelia at 30℃.In terms of fruiting body production,all Lentinus strains demonstrated cultivation potential.The seven Lentinus strains completely colonized the substrate for 18.0-25.6 days and initiated primordia for 21.6-33.5 days.The highest yield(52.5 g bag^(-1))and biological efficiency(10.5%)was recorded in L.strigosus strain 1,whereas the lowest was noted in L.strigosus strain 2 with 21.1 g bag^(-1) yield and 4.2%biological efficiency.L.sajor-caju strain 2 and L.squarrosulus strain 1 showed the widest cap diameter and longest stipe,respectively.The mycelial growth and fruiting body performance of Lentinus may vary depending on species and strains.Altogethe

关 键 词：fruit body formation medicinal mushrooms MYCELIA nutritional requirements physical factors 

分 类 号：R73[医药卫生—肿瘤]