Modulatory effect of D-pinitol on bleomycin-induced pulmonary fibrosis in rats  

作　　者：Yu-Ling Duan Zhi-Hua Wang Yan-Xia Huo Yang Zhang Xiao-Ran Wu Cui-Ke Gong Lin-Lin Bai 

机构地区：[1]Department of Respiration Medicine,Xingtai People’s Hospital,Xingtai,Hebei Province,China

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2023年第5期205-213,共9页亚太热带生物医学杂志（英文版）

摘　　要：Objective:To assess the effect of D-pinitol on pulmonary fibrosis induced by bleomycin.Methods:Sprague-Dawley rats received intratracheal bleomycin(6 IU/kg)to induce pulmonary fibrosis,followed by administration of either D-pinitol(5,10,or 20 mg/kg)or vehicle or methylprednisolone(10 mg/kg)over 28 days after bleomycin administration.Lung function,biochemical parameters,serum biochemistry,mRNA expressions,and histological features were observed.Results:D-pinitol at 10 and 20 mg/kg significantly(P<0.05)attenuated bleomycin-induced bronchoalveolar lavage fluid,decreased myeloperoxidase,nitric oxide,malondialdehyde levels,and increased glutathione and superoxide dismutase level.D-pinitol also improved lung function(enhanced pause,frequency of breathing,expired volume,and tidal volume).Besides,D-pinitol significantly(P<0.05)upregulated Nrf2 and downregulated mRNA expressions of TGF-β,collagen-1,and Smad-3.Furthermore,considerably less inflammation(peribronchial,perivascular,and total),Ashcroft,and interstitial fibrosis scores were observed in the D-pinitol group.Conclusions:D-pinitol exerts its effect against bleomycin-induced pulmonary fibrosis via antioxidative and anti-fibrotic pathways.

关 键 词：ANTIOXIDANT BLEOMYCIN Collagen-1 D-PINITOL Pulmonary fibrosis Smad-3 TGF-Β 

分 类 号：R563[医药卫生—呼吸系统]