Protein S-nitrosylation regulates the energy metabolism of early postmortem pork using the in vitro model  

作　　者：Wenwei Lu Qin Hou Wangang Zhang 

机构地区：[1]Key Laboratory of Meat Processing and Quality Control,Ministry of Education China,Jiangsu Collaborative Innovation Center of Meat Production and Processing,Quality and Safety Control,College of Food Science and Technology,Nanjing Agricultural University,Nanjing 210095,China

出　　处：《Food Materials Research》2022年第1期38-45,共8页食材研究（英文）

基　　金：supported by National Natural Science Foundation of China (Grant No:31871827);China Agriculture Research System of MOF and MARA (CARS-35);the Earmarked Fund for Jiangsu Agricultural Industry Technology System (JATS (2020)425).

摘　　要：This research aimed to investigate the regulation of energy metabolism by protein S-nitrosylation utilizing the in vitro muscle glycolysis model.Longissimus thoracis(LT)muscles homogenates were treated with nitric oxide donor NOR-3((±)-(E)-4-Ethyl-2-(E)-hydroxyimino-5-nitro-3-hexenamide)and control(0.1 M K_(2)HPO_(4))under different pH conditions(6.5,6.0 and 5.5)in vitro buffer system for 24 h,respectively.Results indicated that the NOR-3 treatment group had a significantly higher pH decline rate than the control group and resulted in a higher lactate accumulation and glycogen degradation at 24 h compared with the control group(p<0.05).Moreover,NOR-3 treatment significantly increased the activities along with S-nitrosylation levels of lactate dehydrogenase and glycogen phosphorylase at pH 6.5 in a concentration-dependent manner(p<0.05).In addition,low pH could weaken the NOR-3 treatment effect and inhibit glycolysis rate.Thus,protein S-nitrosylation could play a role in regulating postmortem glycolysis in vitro model even at low pH conditions.

关 键 词：METABOLISM POSTMORTEM TREATMENT 

分 类 号：TS202.1[轻工技术与工程—食品科学]