铁皮石斛多糖超声-微波协同提取条件优化及其抗氧化活性  

Optimization of Ultrasonic-Microwave Synergistic Extraction Conditions of Dendrobium officinale Polysaccharides and Their Antioxidant Activity

作　　者：王林青[1,2] 宁灿灿 李蕊 潘中闪 任红涛 WANG Lin-qing;NING Can-can;LI Rui;PAN Zhong-shan;REN Hong-tao(Zhengzhou Normal University,Zhengzhou 450002,Henan,China;Zhengzhou Nutrition and Health Food Laboratory,Zhengzhou 450002,Henan,China;College of Food Science and Technology,Henan Agricultural University,Zhengzhou 450002,Henan,China)

机构地区：[1]郑州师范学院,河南郑州450002 [2]郑州市营养与健康食品重点实验室,河南郑州450002 [3]河南农业大学食品科学技术学院,河南郑州450002

出　　处：《食品研究与开发》2023年第11期13-20,共8页Food Research and Development

基　　金：郑州市营养与健康食品重点实验室项目(KF20190426)。

摘　　要：以铁皮石斛为原料,采用超声-微波协同法提取铁皮石斛多糖。选择液料比、微波时间、微波功率、超声时间为单因素,以铁皮石斛多糖提取率为评价指标,通过响应面试验对超声-微波协同法提取铁皮石斛多糖进行优化。确定最佳工艺参数为液料比50∶1(mL/g)、微波时间120 s、微波功率370 W、超声时间30 min,在此条件下铁皮石斛多糖提取率为30.56%。根据体外抗氧化活性评价表明,铁皮石斛多糖对羟自由基的清除能力低于维生素C,对α-葡萄糖苷酶表现出明显的抑制能力,具有较好的体外降血糖作用;另外,铁皮石斛多糖对α-淀粉酶也有一定的抑制作用。The Dendrobium officinale polysaccharide was extracted from Dendrobium officinale by ultrasonic-microwave synergistic method.Taken the liquid-solid ratio,microwave time,microwave power and ultrasonic time as single factors,and the extraction rate of Dendrobium officinale polysaccharide as the evaluation index,the ultrasonic-microwave synergistic extraction of polysaccharide from Dendrobium officinale was optimized by response surface method.The optimum process parameters were determined as follows:the liquid-solid ratio was 50∶1(mL/g),the microwave time was 120 s,the microwave power was 370 W,and the ultrasonic time was 30 min.Under this condition,the extraction rate of Dendrobium officinale polysaccharide was 30.56%.According to the evaluation of antioxidant activity in vitro,the scavenging ability of Dendrobium officinale polysaccharides on hydroxyl radicals was lower than that on vitamin C.Dendrobium officinale polysaccharide showed significantα-glucosidase inhibitory activities and had a good in vitro hypoglycemic effect,and it also had a certain inhibitory effect onα-amylase.

关 键 词：铁皮石斛 多糖 超声-微波协同提取 响应面 抗氧化性 

分 类 号：TS201.2[轻工技术与工程—食品科学]