MYBL2基因表达和拷贝数变异与子宫内膜癌患者预后的关系  

Relationship between MYBL2 gene expression and copy number variation and prognosis of patients with endometrial cancer

作　　者：乐露露[1] 傅芬[1] Le Lulu;Fu Fen(Department of Obstetrics and Gynecology,the Second Affiliated Hospital of Nanchang University,Nanchang 330006,China)

机构地区：[1]南昌大学第二附属医院妇产科,江西南昌330006

出　　处：《实用肿瘤杂志》2023年第3期258-264,共7页Journal of Practical Oncology

基　　金：江西省教育厅资助项目(GJJ180078)。

摘　　要：目的探讨成髓细胞瘤转录因子第2亚型(MYB proto-oncogene like 2,MYBL2)基因在子宫内膜癌组织中的表达及拷贝数变异(copy number variation,CNV)情况,评价其与临床特征的关系和对临床预后的影响。方法从癌症基因组图谱(The Cancer Genome Atlas,TCGA)数据库中获取临床病理特征和相应的基因组数据资料完整的子宫内膜癌患者140例,从cBio Cancer Genomics Portal(cBioPortal)数据库下载MYBL2基因CNV和基因组数据资料完整的子宫内膜癌患者137例,以各基因表达水平的中位数作为高低表达的截断值,通过χ2检验和Cox回归分析MYBL2基因高表达及CNV与子宫内膜癌临床特征的关系,Kaplan-Meier曲线分析MYBL2基因表达和CNV与患者预后之间的关系。收集2015年1月至2015年12月南昌大学第二附属医院手术切除经病理组织学确诊的60例子宫内膜癌组织及其癌旁组织。免疫组织化学法检测其MYBL2表达。结果TCGA数据库分析发现,子宫内膜癌MYBL2高表达和低表达患者在病理类型(P=0.034)和组织分级(P<0.01)方面比较,差异均具有统计学意义。cBioPortal数据库分析发现,子宫内膜癌中MYBL2存在CNV(P<0.01),主要变异形式是拷贝数增加,占26.0%。MYBL2 CNV的患者MYBL2 mRNA表达水平上调(P<0.01),MYBL2 CNV患者总生存期与无瘤生存期较短(均P<0.05)。Ⅱ型子宫内膜癌中MYBL2 CNV占比高于Ⅰ型(P<0.01)。结论子宫内膜癌组织中MYBL2呈高表达,且与不良预后相关。MYBL2基因CNV在子宫内膜癌中是一种不良预后因素,其可能成为预测子宫内膜癌临床预后的有效生物标志物。Objective To investigate the expression and copy number variations(CNV)of MYB proto-oncogene like 2(MYBL2)gene in endometrial cancer,and to evaluate its relationship with clinical characteristics and its influence on clinical prognosis.Methods Totally 140 patients with endometrial cancer with complete clinicopathological characteristics and corresponding genomic data were obtained from The Cancer Genome Atlas(TCGA)database,and 137 endometrial cancer patients with complete CNV and genomic data of MYBL2 were downloaded from the cBio Cancer Genomics Portal(cBioPortal)database.The median of each gene expression level was used as the cut-off value of high and low expression.χ2 test and Cox regression were used to analyze the correlation between the high expression and CNV of MYBL2 and the clinical characteristics of endometrial cancer.Kaplan-Meier curve was used to analyze the relationship between the gene expression and CNV of MYBL2 and the prognosis of patients.From January 2015 to December 2015,60 cases of endometrial cancer and its adjacent tissues confirmed by histopathology were collected from the Second Affiliated Hospital of Nanchang University,and MYBL2 expression was detected by immunohistochemistry.Results The TCGA database analysis showed that there were statistically significant differences in pathological type(P=0.034)and histological grade(P<0.01)between patients with high and low expression of MYBL2 in endometrial cancer.cBioPortal database analysis showed that MYBL2 CNV existed in endometrial cancer(P<0.01),and the main form of variation was amplification,accounting for 26.0%.The expression level of MYBL2 mRNA was up-regulated in patients with MYBL2 CNV(P<0.01),and the overall survival and disease-free survival of patients with MYBL2 CNV were shorter(both P<0.05).The proportion of MYBL2 CNV in typeⅡendometrial cancer was higher than that in typeⅠendometrial cancer(P<0.01).Conclusions MYBL2 is highly expressed in endometrial cancer and is associated with poor prognosis.MYBL2 CNV is a poor prognost

关 键 词：子宫内膜癌 成髓细胞瘤转录因子第2亚型 拷贝数变异 预后 

分 类 号：R737.33[医药卫生—肿瘤]