酵母合成环黄芪醇的研究进展  

Advances in the production of cycloastragenol using engineered yeast

作　　者：袁瑛姿 钟泓卉 杨雨潼 姚欢 张晓玲 王峥[3] 魏勇军[1,2] YUAN YingZi;ZHONG HongHui;YANG YuTong;YAO Huan;ZHANG XiaoLing;WANG Zheng;WEI YongJun(School of Pharmaceutical Sciences,Zhengzhou University,Zhengzhou 450001;Laboratory of Synthetic Biology,Zhengzhou University,Zhengzhou 450001;College of Life Science and Technology,Beijing University of Chemical Technology,Beijing 100029,China)

机构地区：[1]郑州大学药学院,郑州450001 [2]郑州大学合成生物学实验室,郑州450001 [3]北京化工大学生命科学与技术学院,北京100029

出　　处：《北京化工大学学报（自然科学版）》2023年第3期14-26,共13页Journal of Beijing University of Chemical Technology(Natural Science Edition)

基　　金：国家自然科学基金(32111530179)。

摘　　要：黄芪是一种重要的中药材,为豆科植物黄芪的干燥根,含有黄酮类、多糖类、皂苷类等多种成分。环黄芪醇主要由黄芪中的黄芪甲苷转化而成,具有抗氧化、抗衰老等多种生物活性。但是现有的环黄芪醇生产策略依赖于中药提取,难以大规模生产。合成生物学技术的快速发展为构建酵母细胞工厂用于高效生产多种植物天然产物提供了基础,也为环黄芪醇的大规模高效合成提供了解决方案。本文综述了现有环黄芪醇的制备方法,对环黄芪醇合成途径及关键酶、萜类合成代谢途径调控等进行了阐述和讨论。未来,通过表达环黄芪醇的关键合成酶基因,并设计和构建酿酒酵母细胞工厂以提高萜类合成代谢通量,有望实现环黄芪醇的酵母合成。Astragali radix is an important Chinese herbal medicine.It is the dried root of Astragalus membranaceus(Fisch.)Bge.var.mongholicus(Bge.)Hsiao or A.membranaceus(Fisch.)Bge.,which contains flavonoids,polysaccharides,saponins and other components.Cycloastragenol is mainly converted from astragaloside in A.radix and has many biological activities such as anti-oxidation and anti-aging.However,current production strategy of cycloastragenol relies on the extraction of traditional Chinese medicine,which is difficult to carry out on a large scale.The rapid development of synthetic biotechnology provides the basis for constructing yeast cell factories to produce a variety of plant natural products with high efficiency and gives a solution for the large-scale and efficient synthesis of cycloastragenol.The available preparation methods of cycloastragenol are reviewed.The synthesis pathway and key enzymes for cycloastragenol and the regulation of the terpenoid synthesis metabolic pathway are described and discussed.In future,it can be expected that yeast synthesis of cycloastragenol will be achieved by expressing the key synthase genes of cycloastragenol and designing and constructing a Saccharomyces cerevisiae cell factory to increase the metabolic flux of terpenoid synthesis.

关 键 词：环黄芪醇 酿酒酵母 微生物细胞工厂 氧化角鲨烯环化酶 细胞色素P450 

分 类 号：Q939.9[生物学—微生物学]