牡蛎鳃部腐败菌的分离鉴定及产胺特性分析  

Isolation and Identification of Putrefactive Bacteria from the Gills of Oysters and Their Characteristics of Amine Production

作　　者：何诗盈 孟鹏 谢飞鸿 苏世京 黄鹭强[1] 陈慧斌 HE Shiying;MENG Peng;XIE Feihong;SU Shijing;HUANG Luqiang;CHEN Huibin(College of Life Sciences,Fujian Normal University,Fuzhou 350117,China;Fujian Inspection and Research Institute for Product Quality,Fuzhou 350002,China)

机构地区：[1]福建师范大学生命科学学院,福建福州350117 [2]福建省产品质量检验研究院,福建福州350002

出　　处：《福建师范大学学报（自然科学版）》2023年第3期78-85,共8页Journal of Fujian Normal University：Natural Science Edition

基　　金：国家自然科学基金资助项目(31401564);闽江学者特聘教授计划启动基金资助项目;福建省科技计划高校产学合作项目(2022N5015)。

摘　　要：为探究牡蛎在腐败过程中产胺菌的种类及产胺特性,通过划线分离和16S rDNA分子鉴定菌株,并采用高效液相色谱检测生物胺质量浓度,结合高通量测序技术分析鳃部微生物的群落组成,.结果表明牡蛎鳃部分离纯化获得的16株菌,分别属于Shewanella、Oceanisphaera、Aeromonas、Marinomonas、Kocuria和Priestia菌属,其中Shewanella和Marinomonas菌属的菌株产胺能力较强.菌株产胺结果表明腐胺、色胺、β⁃苯乙胺和酪胺为牡蛎保藏过程中产胺菌株所产的主要生物胺,而亚精胺、精胺和组胺产量较低.研究结果将为牡蛎优势产胺菌的分离鉴定提供理论基础,并为牡蛎保鲜技术的应用提供科学指导.In order to explore the species and characteristics of amine⁃producing bacteria during oyster spoilage,the bacterial streaking combined with 16S rDNA molecular technology was used to identify the species and further HPLC analysis technology was conducted to detect the mass concentration of biogenic amines.High⁃throughput sequencing technology was used to confirm the microbial community structure of oyster gill.The results showed that 16 isolates were obtained from oyster gill and identified as the genera of Shewanella,Oceanisphaera,Aeromonas,Marinomonas,Kocuria,and Priestia respectively,of which Shewanella and Marinomonas had a strong ability to produce amine.It was concluded that putrescine,tryptamineβ⁃Phenylethylamine and tyramine were the main biogenic amines produced by bacteria isolated from oyster gill during storage,while the spermidine,spermine,and histamine had low concentration during oyster storage.The results of this study will provide a theoretical basis for the isolation and identification of the dominant amine⁃producing bacteria in oysters,and provide scientific guidance for the application of oyster preserva⁃tion technology.

关 键 词：牡蛎 腐败菌 分离鉴定 产胺能力 

分 类 号：TS201.3[轻工技术与工程—食品科学]