Dihydromyricetin Alleviates H9C2 Cell Apoptosis and Autophagy by Regulating CircHIPK3 Expression and PI3K/AKT/mTOR Pathway  

作　　者：ZHANG Zhi-ying LIU Chao WANG Peng-xiang HAN Yi-wei ZHANG Yi-wen HAO Mei-li SONG Zi-xu ZHANG Xiao-ying 

机构地区：[1]Key Laboratory for Molecular Genetic Mechanisms and Intervention Research on High Altitude Disease of Tibet Autonomous Region,School of Medicine,Xizang Minzu University,Xianyang,Shaanxi Province(712082),China [2]Key Laboratory of High Altitude Environment and Gene Related to Disease of Tibet Ministry of Education,School of Medicine,Xizang Minzu University,Xianyang,Shaanxi Province(712082),China [3]School of Finance Economics,Xizang Minzu University,Xianyang,Shaanxi Province(712082),China [4]School of Information Engineering,Xizang Minzu University,Xianyang,Shaanxi Province(712082),China

出　　处：《Chinese Journal of Integrative Medicine》2023年第5期434-440,共7页中国结合医学杂志（英文版）

基　　金：Supported by Science Foundation of Education Department of Shaanxi Provincial Government (No.19JK0890);Natural Science Foundation of Xizang (Tibet) Autonomous Region (No.XZ202001ZR0089G);Major Training Project of Xizang Minzu University (Nos.18MDZ03 and 20MDT03);Funded Project of Young Scholar Cultivation Program of Xizang Minzu University (No.21MDX04)。

摘　　要：Objective: To investigate the effect and potential mechanism of dihydromyricetin(Dmy) on H9C2 cell proliferation, apoptosis, and autophagy. Methods: H9C2 cells were randomly divided into 7 groups, namely control, model, EV(empty p CDH-CMV-MCS-EF1-Cop GFP-T2A-Puro vector), IV(circHIPK3 interference), Dmy(50 μmol/L), Dmy+IV, and Dmy+EV groups. Cell proliferation and apoptosis were detected by cell counting kit-8 assay and flow cytometry, respectivley. Western blot was used to evaluate the levels of light chain 3 Ⅱ/Ⅰ(LC3Ⅱ/Ⅰ), phospho-phosphoinositide 3-kinase(p-PI3K), protein kinase B(p-AKT), and phospho-mammalian target of rapamycin(p-mTOR). The level of circHIPK3 was determined using reverse transcriptase polymerase chain reaction. Electron microscopy was used to observe autophagosomes in H9C2 cells. Results: Compared to H9C2 cells, the expression of circHIPK in H9C2 hypoxia model cells increased significantly(P<0.05). Compared to the control group, the cell apoptosis and autophagosomes increased, cell proliferation rate decreased significantly, and the expression of LC3Ⅱ/Ⅰ significantly increased(all P<0.05). Compared to the model group, the rate of apoptosis and autophagosomes in IV, Dmy, and Dmy+IV group decreased, the cell proliferation rate increased, and the expression of LC3Ⅱ/Ⅰ decreased significantly(all P<0.05). Compared to the control group, the expressions of p-PI3K, p-AKT, and p-mTOR in the model group significantly reduced(P<0.05), whereas after treatment with Dmy and sh-circHIPK3, the above situation was reversed(P<0.05). Conclusion: Dmy plays a protective role in H9C2 cells by inhibiting circHIPK expression and cell apoptosis and autophagy, and the mechanism may be related to PI3K/AKT/mTOR pathway.

关 键 词：DIHYDROMYRICETIN circHIPK AUTOPHAGY cell apoptosis PI3K/AKT/MTOR 

分 类 号：R285[医药卫生—中药学]