基于转录组测序对不同岁龄大青山山羊背最长肌中脂肪代谢相关基因的筛选及分析  

Genes Screening Related to Fat Metabolism Function and Analysis of Daqingshan Goat's(Capra hircus)Longissimus Dorsi Muscle of Different Ages Based on RNA-seq

作　　者：张保军[1] 田建军[1] 张燕军[2] 李金泉[2,3] 李丽杰 贺银凤[1] ZHANG Bao-Jun;TIAN Jian-Jun;ZHANG Yan-Jun;LI Jin-Quan;LI Li-Jie;HE Yin-Feng(College of Food Science and Engineering,Inner Mongolia Agricultural University,Hohhot 010018,China;College of Animal Science,Inner Mongolia Agricultural University,Hohhot 010018,China;Key Laboratory of Mutton Sheep Genetics and Breeding,Ministry of Agriculture,Hohhot 010018,China)

机构地区：[1]内蒙古农业大学食品科学与工程学院,呼和浩特010018 [2]内蒙古农业大学动物科学学院,呼和浩特010018 [3]农业农村部肉羊遗传育种重点实验室,呼和浩特010018

出　　处：《农业生物技术学报》2023年第6期1193-1205,共13页Journal of Agricultural Biotechnology

基　　金：食品科学与工程学院科技计划项目(SPFC202001);内蒙古自治区高等学校科学研究项目(NJZY22491);内蒙古自治区科技计划项目(2019GG243);内蒙古自治区高等学校创新团队发展计划(NMGIRT2322);国家自然科学基金(C170303)。

摘　　要：大青山山羊(Capra hircus)是内蒙古当地典型的肉绒兼用型山羊类群之一。养殖时间、方式等与动物肌肉组织中的脂肪蓄积(或代谢)存在一定关联,而肌肉中的脂肪含量对肉品质产生直接影响。为探索不同养殖阶段大青山山羊肉质的转录组差异及相关差异基因的数据信息,本研究以3个不同岁龄(1.5,2.5和3.5岁)大青山山羊为研究对象,对其背最长肌进行肉质性状指标测定和转录组测序分析。结果表明,3个岁龄组在滴水损失、p H方面差异不显著,3.5岁龄的粗脂肪含量和剪切力显著高于其他两个岁龄组(P<0.05)。转录组数据分析表明,1.5岁龄和2.5岁龄的差异表达基因(转录本)有468个,1.5岁龄和3.5岁龄的差异表达基因(转录本)有487个,2.5岁龄和3.5岁龄的差异表达基因(转录本)有376个,分别富集在44、41和28条代谢通路,3个岁龄组的差异表达基因共富集在85个功能条目,参照GO功能注释和KEGG通路富集分析,经筛选得到G蛋白信号转导调节因子2(regulator of G protein signaling protein 2,RGS2)、雌激素相关受体γ(estrogen-related receptorγ,ESRRG)、过氧化物酶体增殖物激活受体γ辅激活因子1A(peroxisome proliferators-activated receptor-γcoactivator 1 A,PPARGC1A)、肌管蛋白相关蛋白6(myotubularin-related protein 6,MTMR6)、锚蛋白重复结构域成员9(ankyrin repeat domain 9,ANKRD9)、跨膜蛋白160(transmembrane protein 160,TMEM160)、ⅩⅥ磷脂酶A2(groupⅩⅥphospholipase A2,PLA2G16)、FosL1基因抗体(fos-like antigen 1,FOSL1)、金属肽酶含血小板反应蛋白元4(a disintegrin and metalloproteinase with thrombospondin motifs 4,ADAMTS4)共计9个基因差异表达且均参与脂类(包括磷脂)代谢或沉积。随机选择RGS2、ANKRD9、FOSL1和ADAMTS4基因进行qPCR验证,结果显示,4个基因在3个不同岁龄组的表达趋势与转录组测序结果基本一致。本研究获得的差异表达基因可为大青山山羊肉质特性的深入研究提�Daqingshan goat(Capra hircus)is one of the typical meat and cashmere goat groups in Inner Mongolia.There is a certain correlation between the accumulation of fat in muscle or metabolic of farmed animals and breeding time,way and so on.The fat content in the muscle has a direct impact on meat quality.In order to explore the transcriptome differences of meat quality and the data information of related genes in Daqingshan goats at different breeding stages,3 Daqingshan goats of different ages(1.5,2.5 and 3.5 years old)were used as research objects,the meat quality index of the longissimus dorsi was determined and the transcriptome was sequenced and analyzed.Results showed that the 3 age groups had no significant difference in drip loss and pH,but the crude fat content and the shear strength 3.5 years of age were significantly higher than the other 2 age groups(P<0.05).The results of transcriptome data showed that there were 468 differentially expressed genes(transcripts)between 1.5 and 2.5 years old,487 differentially expressed genes(transcripts)between 1.5 and 3.5 years old,and 376 differentially expressed genes(transcripts)between 2.5 years old and 3.5 years old.The differentially expressed genes were enriched in 44,41 and 28 metabolic pathways,respectively,and the differentially expressed genes in 3 age groups were enriched in 85 functional items.According to GO functional annotation and KEGG pathway enrichment analysis,9 differentially expressed genes related to the metabolism or deposition of lipids were obtained.These genes were regulator of G protein signaling protein 2(RGS2),estrogen-related receptorγ(ESRRG),peroxisome proliferators-activated receptor-γcoactivator 1 A(PPARGC1A),myotubularin-related protein 6(MTMR6),ankyrin repeat domain 9(ANKRD9),transmembrane protein 160(TMEM160),XVI phospholipase A2(group XVI phospholipase A2,PLA2G16),fos-like antigen 1(FOSL1)and a disintegrin and metalloproteinase with thrombospondin motifs 4(ADAMTS4).Randomly selected RGS2,ANKRD9,FOSL1 and ADAMTS4 genes were verified b

关 键 词：大青山山羊 岁龄 背最长肌 脂肪沉积 转录组 差异表达基因 

分 类 号：S826[农业科学—畜牧学]