一起金黄色葡萄球菌食物中毒事件的病原检测和溯源分析  

Etiological diagnosis and traceability analysis of a food poisoning caused by Staphylococcus aureus

作　　者：崔琪奇 丁锦 许统圣 任亚萍[1,2] 赵冰 苏靖华[1,2] 王闻卿 黄红 郝莉鹏 潘丽峰[1,2] CUI Qiqi;DING Jin;XU Tongsheng;REN Yaping;ZHAO Bing;SU Jinghua;WANG Wenqing;HUANG Hong;HAO Lipeng;PAN Lifeng(Pudong New District Center for Disease Control and Prevention,Shanghai 200136,China;Pudong Institute of Preventive Medicine,Fudan University,Shanghai 200136,China)

机构地区：[1]上海市浦东新区疾病预防控制中心,上海200136 [2]复旦大学浦东预防医学研究院,上海200136

出　　处：《中国食品卫生杂志》2023年第3期452-457,共6页Chinese Journal of Food Hygiene

基　　金：上海市浦东新区卫生系统优秀青年医学人才培养计划(PWRq2021-12);上海市浦东新区卫生健康委公共卫生高原学科(PWYggy2021-01)。

摘　　要：目的对上海市浦东新区一起食物中毒事件进行病原检测和溯源分析,为此类事件的处置和判定提供依据。方法对病例和从业人员肛拭子以及留样食品样品进行病原分离和鉴定;对分离株进行肠毒素检测、药物敏感性试验、脉冲场凝胶电泳(PFGE)和多位点序列分型(MLST)分子分型和溯源。结果从9份病例肛拭子、1份从业人员肛拭子及3份留样食品样品中分离到13株金黄色葡萄球菌。10株人源分离株产A型和E型肠毒素,3株食源分离株产A型、D型和E型肠毒素。13株分离株均对青霉素耐药,PFGE条带相似度为100%,MLST分型均为ST6型。结论本起事件是由金黄色葡萄球菌污染引起的食物中毒事件,需要进一步加强对食品加工企业、食堂等的宣传教育和管理,防止类似事件的发生。Objective To investigate the etiological features and traceability of a food poisoning incident in Pudong District,Shanghai,and to provide a basis for the future investigation and disposal of similar incidents.Methods Anal swabs of the patients and a chef and food residues were collected for pathogens isolation and identification.Enterotoxin detection immunosorbent assay(ELISA),drug sensitivity test,pulsed field gel electrophoresis(PFGE)and multilocus sequence typing(MLST)were used for genotyping and tracing.Results Nine anal swabs from patients,one anal swab from the chef,and 3 food residues were positive for Staphylococcus aureus(S.aureus).Ten isolates from patients and the chef carried Staphylococcus enterotoxin(SE)A and SEE,while 3 isolates from food carried SEA,SED and SEE.All the isolates were resistant to penicillin.The S.aureus strains from the case shared the same PFGE pattern and the MLST type of them belonged to ST6.Conclusion The cause of this food poisoning outbreak was S.aureus contamination.It is necessary to further strengthen the education and management of food processing enterprises,canteens,etc.to prevent similar incidents.

关 键 词：食物中毒 金黄色葡萄球菌 肠毒素 药敏试验 分子分型 

分 类 号：R155[医药卫生—营养与食品卫生学]