人参皂苷Rb_(1)转化菌株筛选及转化产物分析  

Screening of a strain for ginsenoside Rb1 transformation and analysis of its saponin products

作　　者：李银飞 梁应忠 崔秀明[1,3] 邵琳娇 娄灯吉 杨晓艳 LI Yinfei;LIANG Yingzhong;CUI Xiuming;SHAO Linjiao;LOU Dengji;YANG Xiaoyan(Faculty of Life Science and Technology,Kunming University of Science and Technology,Kunming 650500,Yunnan,China;School of Chemistry,Biology and Environment Sciences,Yuxi Normal University,Yuxi 653100,Yunnan,China;Yunnan Provincial Key Laboratory of Panax notoginseng,Kunming 650500,Yunnan,China)

机构地区：[1]昆明理工大学生命科学与技术学院,云南昆明650500 [2]玉溪师范学院化学生物与环境学院,云南玉溪653100 [3]云南省三七资源可持续发展利用重点实验室,云南昆明650500

出　　处：《微生物学通报》2023年第5期2127-2136,共10页Microbiology China

基　　金：云南省自然科学基金(202001AT070050);国家自然科学基金(32060104)。

摘　　要：【背景】许多微生物能够对皂苷类化合物进行生物转化,因此,通过微生物对皂苷类化合物不同位置结构的修饰能获得高活性的皂苷成分。【目的】从分离纯化的菌株中筛选能将人参皂苷Rb1转化为药理活性较高的稀有人参皂苷。【方法】从三七根际土壤及三七茎中分离纯化了36株真菌,首先利用产β-葡萄糖苷酶的方法对菌株进行皂苷转化活性初筛,再以人参皂苷Rb1为底物进行皂苷转化活性复筛,通过薄层色谱(thin layer chromatography,TLC)、高效液相色谱(high performance liquid chromatography,HPLC)和质谱(mass spectrometry,MS)等方法对转化产物进行分析。【结果】筛选出一株对人参皂苷Rb1具有较高转化活性的菌株F17,通过形态学观察及对内转录间隔区(internal transcribed spacer,ITS)序列分析,菌株F17被鉴定为拟盘多毛孢属菌(Pestalotiopsis biciliata)。P.biciliata可将人参皂苷Rb1转化为人参皂苷Rd、稀有人参皂苷F2和C-K,转化路径为Rb1→Rd→F2→C-K。【结论】本研究为稀有人参皂苷F2和C-K的微生物转化提供了新的菌种来源。[Background]Microbial transformation is an important means for structural modification of natural products.Many microorganisms can transform ginsenoside compounds into saponins with high activity by modifying the structure of different positions.[Objective]To screen a strain that can transform ginsenoside Rb1 into rare ginsenosides with high pharmacological activity.[Methods]A total of 36 fungal strains were isolated from rhizosphere soil and stem of Panax notoginseng and purified.Primary screening of saponin transformation activity was performed withβ-glucosidase method,followed by a second screening with ginsenoside Rb1 as substrate.The transformation products were analyzed by TLC,HPLC,and MS.[Results]A strain F17 with high transformation activity of ginsenoside Rb1 was screened out and it was identified as Pestalotiopsis biciliata based on morphological observation and ITS sequence analysis.It can transform ginsenoside Rb1 into ginsenoside Rd and rare ginsenosides F2 and C-K through the pathway of Rb1→Rd→F2→C-K.[Conclusion]This study provides a new strain for transformation of rare ginsenosides F2 and C-K.

关 键 词：人参皂苷RB1 生物转化 稀有人参皂苷 Β-葡萄糖苷酶 真菌 

分 类 号：TQ925[轻工技术与工程—发酵工程] R284[医药卫生—中药学]