形态发生紊乱关联激活因子l和Fascin在乳腺癌组织中的表达及与临床病理参数和预后的关系研究  

Prognostic expression of disheveled-associated activator of morphogenesis 1 and Fascin in breast cancer and their relationship with clinicopathological parameters and prognosis

作　　者：邢如温 李双进 侯勇强 魏彦 田文娟 Xing Ruwen;Li Shuangjin;Hou Yongqiang;Wei Yan;Tian Wenjuan(Department of General Surgery,Shijiazhuang Sixth Hospital,Shijiazhuang 050011,China;Department of Orthopaedic Surgery,Shijiazhuang Yanzhao Hospital,Shijiazhuang 050035,China;Department of Orthopaedics,Baoding City Yixian Xinglin Hospital,Baoding 074200,China;Department of Surgery,Zhengding County People’s Hospital,Shijiazhuang 050800,China)

机构地区：[1]石家庄市第六医院普外科,050011 [2]石家庄燕赵医院骨外科,050035 [3]保定易县杏林医院骨科,074200 [4]正定县人民医院外科,石家庄050800

出　　处：《中华普通外科学文献（电子版）》2023年第3期211-216,共6页Chinese Archives of General Surgery(Electronic Edition)

基　　金：2021年度河北省医学科学研究课题计划项目(20210949)。

摘　　要：目的 探讨乳腺癌组织中形态发生紊乱关联激活因子l (Daam1)和Fascin表达的相关性及其与临床病理参数和预后的关系。方法 选取2019年1月至2021年12月石家庄市第六医院收治的92例乳腺癌患者作为研究对象。通过免疫组织化学法和实时荧光定量PCR法分别检测乳腺癌组织及相应癌旁组织中Daam1和Fascin蛋白及mRNA表达情况,分析Daam1和Fascin蛋白表达与乳腺癌临床病理参数的关系;Kaplan-Meier法分析Daam1和Fascin表达与乳腺癌预后的关系;Daam1和Fascin表达的相关性采用Spearman秩相关或Pearson相关分析。结果 乳腺癌组织中Daam1、Fascin蛋白阳性率及mRNA表达水平均显著高于癌旁正常组织,差异均有统计学意义(P<0.01)。Daam1阳性表达与乳腺癌远处转移显著相关(P<0.05);Fascin阳性表达与乳腺癌组织低分化、雌/孕激素受体阳性、淋巴结转移及远处转移显著相关(P<0.05)。Daam1和Fascin阳性表达组累积无病生存率均显著低于Daam1和Fascin阴性表达组,差异有统计学意义(Log-rankχ^(2)=3.883、5.159;P=0.049、0.023)。乳腺癌组织中Daam1和Fascin蛋白及mRNA表达均明显正相关(r=0.742、0.689,P<0.05)。结论 Daam1和Fascin在乳腺癌组织中呈正相关性高表达,均与患者临床病理特征及预后密切相关,可能共同参与了乳腺癌的发生、发展及转移。Objective To explore the correlation between disheveled-associated activator of morphogenesis 1(Daam1)and Fascin expression in breast cancer tissues and their relationship with clinicopathological parameters and prognosis.Methods A total of 92 breast cancer patients admitted to Shijiazhuang Sixth Hospital from January 2019 to December 2021 were selected as the research objects.The expression of Daam1 and Fascin proteins and mRNA in breast cancer tissues and corresponding adjacent tissues was detected by immunohistochemistry and quantitative real-time PCR,respectively.The relationship between Daam1 and Fascin protein expression and clinicopathological parameters of breast cancer was analyzed.Kaplan-Meier method was used to analyze the relationship between Daam1 and Fascin expression and breast cancer prognosis.The correlation between Daam1 and Fascin expression was analyzed using Spearman rank correlation or Pearson correlation.Results The positive rates of Daam1 and Fascin protein and mRNA expression levels in breast cancer tissues were significantly higher than those in adjacent normal tissues,and the differences were statistically significant(P<0.01).Positive expression of Daam1 was significantly associated with distant metastasis in breast cancer(P<0.05).Fascin-positive expression was significantly associated with hypodifferentiation,PR positivity,ER positivity,lymph node metastasis and distant metastasis in breast cancer tissues(P<0.05).The cumulative disease-free survival rates in the Daam1 and Fascin-positive expression groups were significantly lower than those in the Daam1 and Fascin-negative expression groups,and the differences were statistically significant(Log-rankχ^(2)=3.883,5.159;P=0.049,0.023).The expression of Daam1 and Fascin protein and mRNA in breast cancer tissues were significantly positively correlated(r=0.742,0.689,both P<0.05).Conclusion Daam1 and Fascin are positively correlated and highly expressed in breast cancer tissues,closely related to the clinicopathological features and prognosi

关 键 词：乳腺肿瘤 Daam1 FASCIN 临床病理参数 预后 

分 类 号：R737.9[医药卫生—肿瘤]