微量元素锌、硒及维生素E对雌性黄鳝生长及抗氧化能力的影响  

Effect of dietary zinc,selenium,and vitamin E on the growth and antioxidant activity of female Monopterus albus

作　　者：岳华梅[1] 符鹏 邓海超 阮瑞[1] 叶欢[1] 李忠[1] 李创举[1] YUE Huamei;FU Peng;DENG Haichao;RUAN Rui;YE Huan;LI Zhong;LI Chuangju(Key Laboratory of Freshwater Biodiversity Conservation,Ministry of Agriculture and Rural Affairs of China/Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Wuhan 430223,China)

机构地区：[1]中国水产科学研究院长江水产研究所,农业农村部淡水生物多样性保护重点实验室,湖北武汉430223

出　　处：《中国水产科学》2023年第3期352-360,共9页Journal of Fishery Sciences of China

基　　金：中国水产科学研究院基本科研业务费专项资助项目(2020XT08).

摘　　要：本研究旨在探讨微量元素锌、硒及维生素E对雌性黄鳝(Monopterus albus)生长、肌肉营养成分及肝脏抗氧化能力的影响。根据正交试验设计L9(34)正交试验,3个因素分别设定3个浓度梯度(锌为20 mg/kg、40 mg/kg、60 mg/kg,硒为0.66 mg/kg、1.31 mg/kg、1.97 mg/kg,维生素E为170 mg/kg、270 mg/kg、370 mg/kg),制备9组等氮等能的饲料。挑选1350尾初始平均体重为(19.67±1.59)g的2冬龄雌性黄鳝,随机分为9组,每组3个重复,每个重复50尾鱼,养殖周期为12周。结果表明:(1)0.66 mg/kg硒添加组黄鳝生长性能显著高于另外两个较高浓度硒添加组(P<0.05),而锌及维生素E添加不影响黄鳝的生长性能(P>0.05),试验组1即0.66 mg/kg硒、20 mg/kg锌、170 mg/kg维生素E添加组雌鳝的生长性能最好,增重率及特定生长率分别为(115.04±8.71)%及(1.39±0.07)%/d;(2)维生素E是影响黄鳝肥满度的首要指标,370 mg/kg维生素E添加组黄鳝的肥满度显著高于170 mg/kg及270 mg/kg添加组(P<0.05);(3)饲料中添加60 mg/kg锌、1.31 mg/kg硒、170 mg/kg维生素E时(试验组8),雌鳝的性腺指数最高,为10.02±1.26;(4)锌为影响雌鳝肌肉粗脂肪含量的第一因素,60 mg/kg锌添加组雌鳝的粗脂肪含量显著高于20 mg/kg及40 mg/kg添加组;(5)60 mg/kg锌添加组黄鳝肝脏的超氧化物歧化酶活性显著高于其他两个添加浓度(P<0.05)。270 mg/kg维生素E添加时雌鳝的丙二醛含量显著低于其他两个添加梯度(P<0.05),而60 mg/kg锌添加时雌鳝的丙二醛含量显著高于其他两个添加梯度(P<0.05)。综上,饲料添加0.66 mg/kg硒、20 mg/kg锌、170 mg/kg维生素E最有利于雌鳝的生长;添加40 mg/kg锌、1.03 mg/kg硒、270 mg/kg维生素E可显著提高雌鳝的抗氧化性能。The aim of this study was to evaluate the influence of dietary zinc,selenium,and vitamin E on the growth and antioxidant activity of female Chinese rice field eels(Monopterus albus).According to the L9(34)orthogonal experiment rule,three different concentrations of zinc(20 mg/kg,40 mg/kg,and 60 mg/kg),selenium(0.66 mg/kg,1.31 mg/kg,and 1.97 mg/kg),and vitamin E(170 mg/kg,270 mg/kg,and 370 mg/kg)were chosen to make nine iso-nitrogenous and iso-energetic diets.A total of 1350 two-year-old female rice field eels with the average body weight of(19.67±1.59)g were selected and randomly allocated into nine groups with three replicates per group and 50 fish per replicate.Fish were fed the nine experimental diets for twelve weeks.The results indicated the following:(1)Female rice field eels grew the best when selenium was added at 0.66 mg/kg,compared to the other two graded concentrations(P<0.05).However,zinc and vitamin E supplements had no significant effect on growth performance(P>0.05).Thus,group 1,with 0.66 mg/kg selenium,20 mg/kg zinc,and 170 mg/kg vitamin E,showed the best growth,with weight gain and specific growth rate values of 115.04±8.71 and 1.39±0.07,respectively.(2)Vitamin E was the main factor influencing the condition factor(CF).The CF value was higher in the 370 mg/kg vitamin E group than in the 170 mg/kg and 270 mg/kg groups(P<0.05).(3)Diets containing 60 mg/kg zinc,1.31 mg/kg selenium,and 170 mg/kg vitamin E(group 8)showed the highest gonadosomatic index,which was 10.02±1.26.(4)The most important determining factor for the crude lipid parameter was zinc,and the group with 60 mg/kg zinc had higher crude lipid than the other two levels of zinc(20 mg/kg and 40 mg/kg)(P<0.05).(5)Fish fed diets containing 60 mg/kg zinc showed higher hepatic superoxide dismutase activity than the other two supplemented level groups(P<0.05).The hepatic malondialdehyde concentration was lower in the 270 mg/kg vitamin E group than in the other two added supplemented level groups(P<0.05),while the 60 mg/kg zinc group had a hi

关 键 词：黄鳝 锌 硒 维生素E 生长 抗氧化能力 

分 类 号：S963[农业科学—水产养殖]