螺旋藻藻蓝色素分离纯化及亚基结构分析  

Purification and Subunit Structure Analysis of Phycocyanin from Spirulina Platensis

作　　者：殷欢 张雅斐 任顺成[1] 李林政 潘天义 YIN Huan;ZHANG Yafei;REN Shuncheng;LI Linzheng;PAN Tianyi(Henan Provincial Key Laboratory of Natural Pigment Preparation,Henan University of Technology,Zhengzhou 450o01,China;Lepu Medical Technology(Beijing)Co.,Ltd.,Beijing 102200,China;Henan Zhongda Hengyuan Biotechnology Co.,Ltd.,Luohe 462600,China)

机构地区：[1]河南工业大学,河南省天然色素制备重点实验室,河南郑州450001 [2]乐普(北京)医疗器械股份有限公司,北京102200 [3]河南中大恒源生物科技股份有限公司,河南漯河462600

出　　处：《食品科技》2023年第4期237-244,共8页Food Science and Technology

基　　金：河南省自然科学基金项目(182300410079)。

摘　　要：为得到高纯度藻蓝色素,采用盐析、透析、羟基磷灰石(Hydroxyapatite HA)柱层析、SDSPAGE等方法对螺旋藻中的藻蓝色素进行了分离纯化及分子质量测定,还对藻蓝色素α亚基和β亚基的结构进行了模拟。经17.5%硫酸铵一步盐析得到藻蓝色素纯度为2.45,50%硫酸铵二步盐析将纯度提高至2.70,再经HA层析得到成品,纯度为4.37;纯化后的样品经SDS-PAGE得到清晰的2条带,分别为α亚基和β亚基,分子质量为17.2 ku和19.7 ku;用SWISS-MODEL和Py MOL对藻蓝色素亚基的结构进行模拟。实验结果可为藻蓝色素的进一步开发利用提供参考。In order to obtain high-purity phycocyanin,the separation,purification and identification procedures of salting out,dialysis,hydroxyapatite column chromatography and SDS-PAGE were established,and the structures of α subunits and β subunits were simulated.The purity of phycocyanin obtained by one-step salting out with 17.5%ammonium sulfate is 2.45,the purity is increased to 2.70 by two-step salting out with 50% ammonium sulfate,and then the reagent grade finished product is obtained by hydroxyapatite column chromatography with a purity of 4.37;After purification,two clear bands were obtained by SDS-PAGE,which were α subunit sum β subunits with molecular weights of 17.2 ku and 19.7 ku;The structure of phycocyanin subunit was simulated by SWISS-MODEL and PyMOL.The results can provide reference for the further development and utilization of phycocyanin.

关 键 词：螺旋藻 藻蓝色素 盐析 纯化 亚基结构 

分 类 号：TS201.2[轻工技术与工程—食品科学]