藻蓝色素对胰岛素抵抗HepG2细胞糖代谢的影响  

Effects of Phycocyanin on Glucose Metabolism in Insulin-Resistant HepG2 Cells

作　　者：李凡念 张文静[1] 郝帅[1] 杨起 李前程 刘媛璞 LI Fannian;ZHANG Wenjing;HAO Shuai;YANG Qi;LI Qiancheng;LIU Yuanpu(School of Food and Health/Beijing Advanced Innovation Center for Food Nutrition and Human Health/Beijing Engineering and Technology Research Center of Food Additives,Beijing Technology and Business University,Beijing 100048,China)

机构地区：[1]北京工商大学食品与健康学院/北京食品营养与人类健康高精尖创新中心/北京市食品添加剂工程技术研究中心,北京100048

出　　处：《食品科学技术学报》2023年第3期54-63,84,共11页Journal of Food Science and Technology

基　　金：国家自然科学基金面上项目(33272321,32072231);北京市高等学校优秀青年人才培养计划项目(BPHR202203042)。

摘　　要：为探究藻蓝色素(phycocyanin,PC)体外改善胰岛素抵抗的作用机制,采用胰岛素体外诱导方式,分别设置5个胰岛素浓度梯度(10-9、10-8、10-7、10-6、10-5μmol/L)以及6个时间梯度(0、12、24、36、48、72 h)处理HepG2细胞,以葡萄糖消耗量和细胞存活率为指标,确定建立胰岛素抵抗型HepG2细胞模型的较佳作用浓度及时间。检测PC干预后HepG2细胞葡萄糖消耗量、糖原合成和存活率,利用RT-qPCR和Western blot探讨PC可能的作用机制。实验结果表明:胰岛素抵抗型HepG2细胞模型最佳诱导时间和浓度为36 h、10-7μmol/L;不同浓度的PC可以提升胰岛素抵抗型HepG2细胞的葡萄糖消耗量;PC能够上调正常HepG2和胰岛素抵抗型HepG2细胞中IRS1、IRS2、GLUT1、GLUT4基因的转录水平,并且增加细胞中IRS1、AMPK、GSK-3β以及AKT蛋白的磷酸化水平。研究结果表明,PC能够显著提升胰岛素抵抗型HepG2细胞的葡萄糖消耗量,通过激活胰岛素调节相关的IRS1/AKT信号通路,增加AMPK的磷酸化和葡萄糖转运蛋白GLUT1、GLUT4基因表达,加速HepG2细胞对葡萄糖的利用和改善细胞胰岛素抵抗。研究结果显示,PC在预防或改善2型糖尿病肝脏胰岛素抵抗方面有潜在作用。To investigate the mechanism of phycocyanin(PC)on improving insulin resistance(IR)in vitro,high concentration of insulin was used to induce IR model.HepG2 cells were treated with five insulin concentration gradients(10-9,10-8,10-7,10-6,10-5μmol/L)and six time gradients (0, 12, 24, 36, 48, 72 h), respectively. According to the results of glucose consumption and cell viability, the optimal concentration and time of insulin resistance model of HepG2 (IR-HepG2) cells were determined. After PC intervention, glucose consumption, glycogen synthesis and cell viability of normal and IR-HepG2 cells were detected, respectively. RT-qPCR and Western blot were used to investigate the possible mechanism. The results showed that IR-HepG2 cells was optimally established with 10 -7 μmol/L insulin treatment for 36 h. Different concentrations of PC could promote glucose consumption of IR-HepG2 cells. PC could up-regulate the transcription levels of IRS1, IRS2, GLUT1 and GLUT4 genes in both normal and IR-HepG2 cells, and increased the phosphorylated protein expression levels of IRS1, AMPK, GSK-3β and AKT. PC might significantly promote glucose consumption in IR-HepG2 cells by activating IRS1/AKT signaling pathway and increasing the phosphorylation of AMPK, also activating expression of GLUT1 and GLUT4 genes ecoding glucose transporter,and accelerate glucose utilization and improve insulin resistance in IR-HepG2 cell. This study indicated that PC had a potential role in preventing or improving hepatic insulin resistance of type 2 diabetes mellitus.

关 键 词：藻蓝色素 HEPG2细胞 糖代谢 AKT 胰岛素抵抗 

分 类 号：TS202.3[轻工技术与工程—食品科学] R151.1[轻工技术与工程—食品科学与工程]