儿茶素经PI3K-Akt-eNOS信号通路对冠心病大鼠心肌损伤及抗炎作用分析  

Analysis of Myocardial Injury and Aanti-inflammatory Effect of Catechinin Coronary Heart Disease Rats through PI3K-Akt-eNOS Signaling Pathway

作　　者：张文强 文亮 李慧 叶飞林 薛强 ZHANG Wen-qiang;WEN Liang;LI Hui;YE Fei-lin;XUE Qiang(Department of Cardiology,986 Air Force Hospital,Xian,Shaanxi,710054,China;Department of Cardiology,Xijing Hospital of Air Force Medical University,Xi'an,Shaanxi,710032,China)

机构地区：[1]空军第九八六医院心内科,陕西西安710054 [2]空军军医大学西京医院心内科,陕西西安710032

出　　处：《现代生物医学进展》2023年第9期1619-1623,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金青年项目(81600356)。

摘　　要：目的:探讨儿茶素经PI3K-Akt-eNOS信号通路对冠心病大鼠心肌损伤及抗炎作用分析。方法:健康成年SPF级SD雄性大鼠,经腹腔注射垂体后叶素构建冠心病模型。采用Western blot法检测大鼠PI3K-Akt-eNOS信号通路相关蛋白的表达情况。采用ELISA法检测大鼠炎症因子和氧化应激指标的表达。观察并记录大鼠心肌损伤情况。结果:与空白组相比,模型组、儿茶素小剂量组、儿茶素中剂量组、儿茶素大剂量组的心肌缺血和心肌梗死面积均明显更高(P<0.05);儿茶素大剂量组心肌缺血和心肌梗死面积明显小于模型组、儿茶素小剂量组、儿茶素中剂量组(P<0.05);与空白组相比,模型组、儿茶素小剂量组、儿茶素中剂量组、儿茶素大剂量组的PI3K、p-Akt/Akt、p-eNOS/eNOS均明显更高(P<0.05);儿茶素大剂量组PI3K、p-Akt/Akt、p-eNOS/eNOS明显高于模型组、儿茶素小剂量组、儿茶素中剂量组(P<0.05);与空白组相比,模型组、儿茶素小剂量组、儿茶素中剂量组、儿茶素大剂量组的TNF-α、IL-1β、IL-18均明显更高(P<0.05);儿茶素大剂量组TNF-α、IL-1β、IL-18明显低于模型组、儿茶素小剂量组、儿茶素中剂量组(P<0.05);与空白组相比,模型组、儿茶素小剂量组、儿茶素中剂量组、儿茶素大剂量组的ROS、GSH-Px、MDA均明显更高(P<0.05);儿茶素大剂量组TNF-α、IL-1β、IL-18明显低于模型组、儿茶素小剂量组、儿茶素中剂量组(P<0.05)。结论:儿茶素可通过PI3K-Akt-eNOS信号通路靶向改善冠心病大鼠心肌炎症和氧化应激状况,进而发挥心肌保护作用。Objective:To investigate the effects of catechin on myocardial injury and anti-inflammatory effects in rats with coronary heart disease through PI3K-Akt-eNOS signaling pathway.Methods:Healthy adult SPF-grade SD male rats were injected with pituitrin intraperitoneally to establish coronary heart disease model.The expression of PI3K-Akt-eNOS signaling pathway was detected by Western blot.The expressions of inflammatory factors and oxidative stress were detected by ELISA.Myocardial injury in rats was observed and recorded.Results:Compared with blank group,myocardial ischemia and myocardial infarction area in model group,catechin low-dose group,catechin medium-dose group and catechin high-dose group were higher(P<0.05).The area of myocardial ischemia and myocardial infarction in catechin high-dose group was smaller than that in model group,catechin low-dose group and catechin medium-dose group(P<0.05).Compared with blank group,PI3K,p-Akt/Akt and P-ENOS/eNOS in model group,catechin low-dose group,catechin medium-dose group and catechin high-dose group were higher(P<0.05).PI3K,P-Akt/Akt and P-ENOS/eNOS in catechin high-dose group were higher than those in model group,catechin low-dose group and catechin medium-dose group(P<0.05);Compared with blank group,TNF-α,IL-1βand IL-18 in model group,catechin low-dose group,catechin medium-dose group and catechin high-dose group were higher(P<0.05).TNF-α,IL-1βand IL-18 in catechin high-dose group were lower than those in model group,catechin low-dose group and catechin medium-dose group(P<0.05).Compared with blank group,ROS,GSH-Px and MDA in model group,catechin low-dose group,catechin medium-dose group and catechin high-dose group were higher(P<0.05).TNF-α,IL-1βand IL-18 in catechin high-dose group were lower than those in model group,catechin low-dose group and catechin medium-dose group(P<0.05).Conclusions:Catechin can improve myocardial inflammation and oxidative stress in rats with coronary heart disease by targeting the PI3K-Akt-eNOS signaling pathway,and thus play a

关 键 词：儿茶素 PI3K-Akt-eNOS 冠心病 

分 类 号：R-33[医药卫生] R541.4