Evolving a novel red-emitting two-photon dye with optically tunable amino group for monitoring the degree of hypoxia during liver fibrosis  

作　　者：Xingxing Zhang Feiyu Yang Tianbing Ren Yingxin Zheng Xiao-Bing Zhang Lin Yuan 

机构地区：[1]State Key Laboratory of Chemo/Biosensing and Chemometrics,College of Chemistry and Chemical Engineering,Hunan University,Changsha 410082,China

出　　处：《Chinese Chemical Letters》2023年第5期480-483,共4页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos.22074036,22004033,21877029);Special Funds for the Construction of Innovative Provinces in Hunan Province(No.2019RS1031)。

摘　　要：Two-photon imaging has attracted increasing attention owing to its deep tissue imaging capabilities.Therefore,many fluorophores have been developed to satisfy its requirements.However,long-wavelength emission fluorophores with an optically tunable group are rarely developed.In this study,two longwavelength emission fluorophores with an optically tunable amino group were successfully developed by introducing strong electron acceptor and large conjugated group to the TPQL dye.TPCO_(2)displayed a bright red emission(λem=638 nm,Φ=0.15)together with high two-photon action cross section and good water solubility,which enabled higher signal-to-background ratios and deep tissue imaging.The proof-of-concept probe(TPCO-NO_(2))was successfully applied to the high signal-to-background ratio imaging of nitroreductase in liver fibrosis,further realizing diagnosis of the degree of hypoxia during liver fibrosis.

关 键 词：Fluorescent probes Two-photon imaging FLUOROPHORES NITROREDUCTASE HYPOXIA 

分 类 号：O657.3[理学—分析化学] TQ617[理学—化学] R575.2[化学工程—精细化工]