食用油过氧化值显色试纸的制备与快速测定  

The Establishment of Read-Out Visualization Test Paper for Edible Oil Peroxide Value and Rapid Analysis

作　　者：何连军[1] 张宜明[2] HE Lianjun;ZHANG Yiming(Hangzhou Vocational and Technical College,Hangzhou,Zhejiang 310018;College of Food and Health,Zhejiang A&F University,Hangzhou,Zhejiang 311300)

机构地区：[1]杭州职业技术学院,浙江杭州310018 [2]浙江农林大学食品与健康学院,浙江杭州311300

出　　处：《核农学报》2023年第6期1227-1234,共8页Journal of Nuclear Agricultural Sciences

基　　金：杭州市农业与社会发展计划项目(20191203B26);浙江省基础公益研究计划(LGF22C200001)。

摘　　要：为建立新型试纸比色方法以精确快速测定油脂的过氧化值,本研究通过溶胶-凝胶法将二价铁离子以纳米复合物形式构筑在滤纸表面,然后利用油脂的过氧化性将二价铁离子转化为三价铁离子,并使其与二甲酚橙(XO)反应生成橙红色配合物。通过比较滤纸上显色的深浅来表示油脂过氧化值的高低。结果表明,通过优化试验参数,油脂的过氧化值与显色深浅呈现相关关系。进一步使用智能手机拍照并通过ImageJ软件图像识别模式,可以初步半定量检测实际油脂样品,相关系数可达到0.9852,最低检出限可以达到0.2 mmol·kg^(-1),从而实现快速便捷的油脂过氧化值直读测定。综上,以二甲酚橙为显色剂稳定性好,铁离子纳米二氧化硅复合材料有助于构筑稳定的油脂过氧化值试纸显色体系。本研究建立的试纸为现场快速检测油脂过氧化值提供了一种新方法。In order to establish a new paper colorimetric method for accurate and rapid determination of lipid peroxide value.In this study,ferrous ion was firstly constructed on the surface of filter paper in the form of nano composite via sol-gel method.Then,the ferrous ions can be converted to ferric ions to form orange-red complexes with xylenol orange(XO)by using the peroxidation property of the oil.The peroxide value of edible oil is indicated by the depth of color on filter paper.After the optimization of the experimental parameters,the peroxide value of the oil is closely related to the color depth,and the peroxide value of the oil can be directly judged according to the color depth.Furthermore,based on the image recognition mode of ImageJ software,the actual oil samples can be semi-quantitative detected.The rapid and convenient direct reading determination of oil peroxide value can be achieved that the correlation coefficient can reach 0.9852,and the minimum detection limit can reach 0.2 mmol·kg^(-1).It is found that xylenol orange as the color developing agent has good stability and iron ion nano silica composite is helpful to construct stable oil peroxide value test paper color developing system.The paper provides a new method for the rapid detection of oil peroxide value on situ.

关 键 词：食用油 过氧化值 二甲酚橙 试纸 快速测定 

分 类 号：TS227[轻工技术与工程—粮食、油脂及植物蛋白工程]