饲料中茶多酚的添加量对团头鲂幼鱼生长、饲料利用和抗氧化能力的影响  

Effects of different supplementation of dietary tea polyphenols on growth performance,feed utilization and antioxidant capacity of Megalobrama amblycephala

作　　者：胡颂钦 穆俏俏 林艳[2] 缪凌鸿[1,2] 刘波[1,2] 董在杰[1,2] HU Songqin;MU Qiaoqiao;LIN Yan;MIAO Linghong;LIU Bo;DONG Zaijie(Wuxi Fisheries College,Nanjing Agricultural University,Wuxi 214081,China;Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization,Ministry of Agriculture and Rural Affairs,Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi 214081,China)

机构地区：[1]南京农业大学无锡渔业学院,江苏无锡214081 [2]中国水产科学研究院淡水渔业研究中心,农业农村部淡水渔业和种质资源利用重点实验室,江苏无锡214081

出　　处：《水产学报》2023年第6期142-153,共12页Journal of Fisheries of China

基　　金：现代农业产业技术体系国家大宗淡水鱼产业技术体系(CARS-45)。

摘　　要：为研究饲料中添加茶多酚对团头鲂幼鱼生长性能、饲料利用和抗氧化能力的影响,选取240尾初始体重为(3.5±1.0) g的团头鲂幼鱼,随机分为4组,每组3个重复,每个重复20尾。实验配制了茶多酚添加量分别为0 (对照组)、100、300和500 mg/kg的等氮等能饲料,饲喂团头鲂幼鱼8周后测定鱼体生长性能、肌肉组成、血液生化指标和肝肠抗氧化指标。结果显示,摄食300 mg/kg茶多酚组的增重率(WGR)相较于对照组显著提高,蛋白质效率(PER)显著高于低添加量组,饲料系数(FCR)显著降低。500 mg/kg茶多酚显著降低了鱼体肌肉粗脂肪含量。茶多酚的添加显著降低了血浆葡萄糖(GLU)含量,且300和500 mg/kg茶多酚添加组团头鲂肝肠丙二醛(MDA)含量均显著低于无添加组。100和300mg/kg茶多酚添加组鱼体肝肠组织中还原性谷胱甘肽(GSH)含量显著高于对照组。此外,300 mg/kg茶多酚添加组鱼体肝脏的总抗氧化能力(T-AOC)及谷胱甘肽过氧化物酶(GSHPx)活性也显著高于对照组。养殖实验结束后,通过腹腔注射浓度为50%的四氯化碳(CCl_(4))溶液诱导急性肝应激损伤,发现注射后24 h,300和500 mg/kg茶多酚组存活率均显著高于100 mg/kg茶多酚组,且100 mg/kg茶多酚添加组显著高于对照组。进一步分析注射浓度为50%的CCl_(4)溶液24 h后鱼体肝脏抗氧化指标,结果显示,茶多酚的添加显著降低了急性肝损伤后团头鲂肝脏MDA含量,500 mg/kg茶多酚添加组肝脏超氧化物歧化酶(SOD)活性显著高于对照组,且300 mg/kg茶多酚添加组肝脏GSH含量显著高于对照组。研究表明,团头鲂幼鱼饲料中添加300 mg/kg茶多酚能有效促进团头鲂幼鱼生长和饲料利用,提高肝肠抗氧化能力;茶多酚添加量为500 mg/kg具有保护团头鲂抵抗氧化应激损伤的作用。本研究结果为茶多酚在团头鲂饲料中的研究提供依据,为其更好地在养殖生产中的应用提供参考。In recent years,the development of antibiotic substitutes with the functions of immune promotion and vegetative growth is the development trend of aquaculture industry.In order to study the effect of dietary tea polyphenols(TP)on the growth performance,feed utilization and antioxidant ability of Megalobrama amblycephala,in the experiment,240 M.amblycephala with an initial weight of(3.5±1.0)g were selected and randomly divided into 4 groups of 3 replicates,each with 20 fish in each replicate.Four isonitrogen isoenergetic formulation diets supplemented with 0 mg/kg(control group),100,300 and 500 mg/kg tea polyphenols were prepared.After the 8-week feeding trial,the growth performance,muscle composition,blood biochemical indicators,liver antioxidant indicators and intestinal antioxidant indicators were measured.The results showed that the WGR of the fish fed 300 mg/kg TP were significantly higher than those of the control group,the PER was significantly higher than those with lower supplementary TP,and the FCR decreased significantly.500 mg/kg TP supplementation significantly reduced the crude fat content of fish muscle.The TP supplementation significantly reduced plasma GLU content.The content of MDA in the liver and intestines of fish fed 300 mg/kg and 500 mg/kg TP supplementary diets were significantly lower than those in the control group.The content of reduced GSH in the liver and intestine tissues of fish fed 100 mg/kg and 300 mg/kg dietary TP were significantly higher than that in the control group.In addition,the hepatic T-AOC and GSH-Px activity of the 300 mg/kg TP supplementary group were also significantly higher than those in the control group.After the feeding trial,acute liver injury was induced by intraperitoneal injection of 50%concentration of CCl_(4) solution.It was found that at 24 hours post-injection,the fish survival rates of the 300 mg/kg and 500 mg/kg TP supplementary groups were significantly higher than those of the 100 mg/kg TP supplementary group,and the survival rates in 100 mg/kg TP su

关 键 词：团头鲂 茶多酚 生长性能 抗氧化能力 肝损伤 

分 类 号：S963.73[农业科学—水产养殖]