小鼠幼年期听力剥夺导致成年期社交障碍  

Hearing deprivation in early period causes impaired social competence in adult mice

作　　者：周学华 金琳 沈霞 ZHOU Xue-hua;JIN Lin;SHEN Xia(Department of Anesthesiology,Eye and ENT Hospital,Fudan University,Shanghai 200031,China)

机构地区：[1]复旦大学附属眼耳鼻喉科医院麻醉科,上海200031

出　　处：《复旦学报（医学版）》2023年第3期419-424,共6页Fudan University Journal of Medical Sciences

基　　金：国家自然科学基金(81671045,82171184)。

摘　　要：目的观察小鼠幼年期听力剥夺对其成年期社交能力的影响。方法新生昆明小鼠被随机分成对照组(n=36)和听力剥夺组(n=32)。听力剥夺组小鼠在出生后第7天开始连续1周予以皮下注射新霉素200 mg/kg;对照组则予以注射同等剂量的生理盐水。在1月龄和3月龄分别采用听觉脑干反应(auditory brainstem response,ABR)进行听力测试。在小鼠3月龄时,采用三箱社交实验检测社交能力、旷场实验检测焦虑程度以及Y迷宫检测学习记忆能力。结果新生鼠新霉素皮下注射导致持久的听力损害。与对照组相比,听力剥夺组3月龄时小鼠社交互动时间缩短[(64.89±9.58)s vs.(107.20±39.18)s,P<0.05]和社交记忆损害;听力剥夺组小鼠与1号陌生小鼠的社交时间与2号陌生小鼠社交时间相当[83.28(40.32~85.72)s vs.57.48(45.56~64.36)s,P>0.05];旷场实验中在中心区域逗留时间较短[157.00(57.68~180.20)s vs.202.50(169.80~230.80)s,P<0.05];在Y迷宫中正确的轮替率较低(47.23%±11.81%vs.61.28%±7.70%,P<0.05)。结论小鼠幼年期听力剥夺可导致其成年期社交能力下降、焦虑程度增加和学习记忆能力受损,提示完整的听力功能是保持正常认知功能的重要前提条件。Objective To identify the relationship between hearing deprivation and social skills.Methods The mice were randomly divided into control group(n=36)and hearing deprivation group(n=32).On postnatal day 7(P7),the experimental mice received one daily neomycin dose for 7 consecutive days(200 mg/kg,subcutaneous injection),and the corresponding dose of sterile saline was separately injected into the control group mice.The auditory brainstem response test(ABR)was applied to compare hearing thresholds of mice at the age of 1 month and 3 month.In mice at three months of age,three-chamber social interaction test was used to evaluate the social ability,Open field experiment was used to evaluate the anxiety level,and Y maze were used to evaluate the learning and memory function.Results Neomycin administration to mice in early period caused long lasting and severe hearing loss in adulthood.Compared with the controls,mice with hearing deprivation showed impaired social interaction capability[(64.89±9.58)s vs.(107.20±39.18)s,P<0.05]and social memory the hearing loss mice spent comparable time with strange mouse 1 and strange mouse 2[83.28(40.32-85.72)s vs.57.48(45.56-64.36)s,P>0.05]in the 3-chambered social test,elevated anxiety level in the open field test[157.00(57.68-180.20)s vs.202.50(169.80-230.80)s,P<0.05],and impaired learning and memory function in the Y maze test(47.23%±11.81%vs.61.28%±7.70%,P<0.05).Conclusion Our study demonstrated that hearing deprivation in early period in mice may lead to impaired social competence,elevated anxiety level and impaired learning and memory function.The findings support that hearing input is required for the development of hippocampal dependent normal cognitive function.

关 键 词：听力剥夺 社交能力 焦虑 认知功能 海马 小鼠 

分 类 号：R-332[医药卫生]