物理诱变和高通量筛选在益生菌选育中的应用  

Application of Physical Mutagenesis and High Throughput Screening Technology in the Selection of Probiotics

作　　者：潘丽娜 唐溶雪 康文丽 李意思 胡鹏钰 汪家琦 周洪波[1,4] PAN Lina;TANG Rongxue;KANG Wenli;LI Yisi;HU Pengyu;WANG Jiaqi;ZHOU Hongbo(School of Minerals Processing and Bioengineering,Central South University,Changsha 410083,China;Ausnutria Dairy(China)Co.,Ltd.,Changsha 410127,China;Ausnutria Food and Nutrition Research Institute,Changsha 410299,China;Hunan Engineering Research Center of Human Microecological Products,Changsha 410017,China)

机构地区：[1]中南大学资源加工与生物工程学院,湖南长沙410083 [2]澳优乳业(中国)有限公司,湖南长沙410127 [3]湖南澳优食品与营养研究院,湖南长沙410299 [4]人体微生态制品湖南省工程研究中心,湖南长沙410017

出　　处：《食品工业科技》2023年第13期458-465,共8页Science and Technology of Food Industry

基　　金：湖南省创新平台与人才计划“湖南省营养健康品工程技术研究中心”项目(2019TP2066)。

摘　　要：野生型菌株活性较低,难以满足工业化需求,通过使用物理诱变方法可改善菌种性能,进而获得高产、优质菌株。同时需寻找快速、合适的筛选方法从突变文库中获得理想目标菌株。传统人工筛选及摇瓶培养成本高、耗时耗力,高通量筛选技术解决了这一难题。本文探讨了传统及新型物理诱变技术原理,对比了两类诱变技术的区别及阐述了其在益生菌选育中的应用。同时总结了各高通量筛选技术(微量滴定板筛选、荧光激活细胞分选、生物传感器的筛选、液滴微流控平台筛选及模式动物平台的筛选)的特点及其在益生菌筛选的相关应用。本文为后续降低筛选成本、提高筛选效率、获得高产理想目标菌株提供重要参考。Wild-type strains hardly meet the current industrial demands due to low stability.The performance of microbial strains(high-yielding,high-quality strains)can be improved by using physical mutagenesis techniques.At the same time,high throughput screening methods and techniques can be used to quickly obtain ideal strains from the mutation library.However,traditional manual screening and shaker culture are high-cost,time-consuming,and laborious,and high-throughput screening technology can solve this problem.In this review,the applications of traditional and novel mutagenesis used for the improvement of probiotics are summarized,the principles of traditional and novel physical mutagenesis techniques are discussed,the differences between the two kinds of mutagenesis techniques are compared and their applications in the improvement of probiotics are described.At the same time,the characteristics and relevant applications of various high-throughput screening technologies(microtitration plate screening,fluorescence activated cell sorting, biosensors screening, droplet microfluidic platform screening and model animal platform screening) are summarized. This review provides important reference for reducing screening cost, improving screening efficiency and obtaining high yield ideal target strains.

关 键 词：物理诱变 高通量筛选 益生菌 应用 

分 类 号：TS201.3[轻工技术与工程—食品科学]