超声波辅助提取黄绿蜜环菌多糖的工艺研究  

Study on the Ultrasonic-assisted Extraction of Polysaccharides from Armillaria luteo-virens

作　　者：杜亚飞 李维 但玉兰 张茂碟 DU Yafei;LI Wei;DAN Yulan;ZHANG Maodie(College of Chemistry and Life Sciences,Chengdu Normal University;Sichuan Provincial Key Laboratory for Development and Utilization of Characteristic Horticultural Biological Resources,ChengDu SiChuan,611130,China)

机构地区：[1]成都师范学院化学与生命科学学院 [2]特色园艺生物资源开发与利用四川省高等学校重点实验室,四川成都611130

出　　处：《高原农业》2023年第3期298-304,共7页Journal of Plateau Agriculture

基　　金：成都师范学院校级科研项目(CS21ZCY03);超声波辅助提取黄绿蜜环菌多糖的工艺研究。

摘　　要：本研究旨在优化超声波辅助提取黄绿蜜环菌子实体多糖的最佳工艺条件。以黄绿蜜环菌多糖提取率为指标,利用单因素试验考察超声温度、超声时间、超声功率以及料液比对提取率的影响,在此基础上进行正交设计优化,并与传统热水浴浸提法的多糖提取率进行比较。结果显示,当超声温度为60℃,超声时间为40 min,超声功率为195 W,料液比为1:20 g/mL的条件下,黄绿蜜环菌多糖提取率为7.93%。研究表明,与传统热水浴浸提法相比,超声波辅助提取法显著减少了多糖的提取时间,并且有效地提高了多糖提取率。In this paper,the optimum process conditions of ultrasonic-assisted extraction of polysaccharides from Armillaria luteo-virens were studied.The extraction rate of polysaccharide from Armillaria luteo-virens was taken as the index,and effects of ultrasonic temperature,ultrasonic time,ultrasonic power and solid-liquid ratio on the extraction rate of polysaccharide were investigated by single factor experiment;on this basis,orthogonal test was carried out and the extraction rate of polysaccharide was compared with that of traditional hot-water-bath extraction method.The results indicated that when the ultrasonic temperature was 60℃;ultrasonic time was 40 min;ultrasonic power was 195W;solid-liquid ratio was 1:20 g/mL,the extraction rate of polysaccharide was 7.93%.Compared with the traditional hot-water-bath extraction method,the ultrasonic-assisted extraction method reduced the extraction time of polysaccharides significantly and improved the extraction rate effectively.

关 键 词：黄绿蜜环菌 超声辅助提取 多糖 正交试验 

分 类 号：TS201.3[轻工技术与工程—食品科学]