基于免疫脂质体的荧光分析法检测棒曲霉素  

An Immunoliposome-based Fluorescence Assay for the Detection of Patulin

作　　者：宋新杰 吕天凤 吴丽 孙娟 张尧 金明姬 吴元锋 Song Xinjie;LüTianfeng;Wu Li;Sun Juan;Zhang Yao;Kim Myunghee;Wu Yuanfeng(Zhejiang Provincial Key Laboratory of Chemical and Biological Processing Technology for Agricultural Products,School of Biological and Chemical Engineering,Zhejiang University of Science and Technology,Hangzhou 310023;Yeungnam University,Gyeongsan 38541,Korea)

机构地区：[1]浙江科技学院生物与化学工程学院,浙江省农产品化学与生物加工技术重点实验室,杭州310023 [2]岭南大学,韩国庆山38541

出　　处：《中国食品学报》2023年第5期381-386,共6页Journal of Chinese Institute Of Food Science and Technology

基　　金：浙江省重点研发计划项目(2021C02061)。

摘　　要：棒曲霉素是一种霉菌产生的天然毒素,曲霉菌、丝衣霉菌、青霉菌、毛霉和根霉等真菌均可产生,能污染水果、蔬菜、谷物和奶酪等多种食物,对食品安全造成威胁。传统的棒曲霉素检测方法需用有机溶液进行分离和浓缩,对环境产生一定的压力。针对传统方法的缺陷,本文利用兔抗棒曲霉抗体及其修饰的脂质体开发一种快速、灵敏、环境友好的荧光检测方法,检测限可达8μg/L,低于欧洲婴儿和幼儿食品监管要求(10μg/L)。本方法快速、灵敏、环境友好,可用于苹果汁等食品中棒曲霉毒素的检测。Patulin(4-hydroxy-4H-fruo[3,2-c]pran-2[6H]-one)is a naturally occurring mycotoxin produced by a number of fungal including Aspergillus,Byssochlamys,Penicillium,Mucor,and Rhizopus,and contaminate a wide variety of food products such fruit products,vegetables,grains and cheeses.The conventional method for patulin detection required separation and concentration process with organic solvent which is environmentally harmful.Considering the limitation of the traditional methods,this study developed a rapid,sensitive and environmentally friendly method for detection of patulin by using of a rabbit antibody against patulin and antibody conjugated immunolipsome.The detection limit of developed assay had detection limit of 8μg/L without the need of organic solvent for extraction,separation,and purification.Overall,the detection limit meet well of European regulatory limit for infants and young children products(10μg/L).The developed method was rapid,sensitive,environment-friendly,and reliable for the detection of patulin in apple juice.

关 键 词：棒曲霉素抗体 免疫脂质体 棒曲霉素 灵敏 快速检测 

分 类 号：TS207.5[轻工技术与工程—食品科学] O657.3[轻工技术与工程—食品科学与工程]