乳酸菌发酵中药对大鼠痛风症状的影响研究  

Study on effect of lactic acid bacteria on gout symptoms in rats

作　　者：陈妍 李市场[1] 杜莹瑞 胡锋阳 丁慧 龚明贵[1] CHEN Yan;LI Shi-chang;DU Ying-rui;HU Feng-yang;DING Hui;GONG Ming-gui

机构地区：[1]河南科技大学食品与生物工程学院,河南洛阳471000

出　　处：《饲料研究》2023年第10期79-84,共6页Feed Research

基　　金：国家自然科学基金面上项目(项目编号:31870093)。

摘　　要：试验以马齿苋、苦荞等混合植物为底物,探讨中药发酵液成分对痛风性关节炎大鼠血清生化指标及肾脏、踝关节滑膜组织病理形态的影响。采用UPLC-MC/MC技术对中药发酵液的成分分析,选取60只雄性昆明大鼠随机分为6组,每组10只大鼠。空白对照组大鼠饲喂正常饲料,其余组全部饲喂2%氧嗪酸钾联合12%酵母膏特殊饲料进行造模。4周后,空白对照组和模型对照组灌胃生理盐水,低浓度发酵液组、中浓度发酵液组、高浓度发酵液组分别灌胃20%、40%、60%中药发酵液,灌胃量为16 mL/kg。试验期3 w。结果显示,中药发酵液中黄酮类化合物包括55种,相对含量占15.39%。在动物试验中,与模型对照组相比,高浓度发酵液组大鼠血清尿酸(UA)、尿素氮(BUN)、肌酐(Cr)和肿瘤坏死因子-α (TNF-α)含量均极显著降低(P<0.01)。肾脏和踝关节滑膜组织病理切片显示,中药发酵液可改善肾组织损伤和痛风性关节炎大鼠脚踝炎症。研究表明,中药发酵液对由高尿酸引起的肾损伤具有一定的保护作用,对痛风性关节炎大鼠具有一定的消炎作用,以高浓度效果最为明显。The study used mixed plants such as Purslane and Tartary buckwheat as substrates,to investigate the effect of fermented Chinese medicine liquid on serum biochemical indexes and pathological morphology of kidney and ankle synovium in gouty arthritis rats.Sixty male Kunming rats were randomly divided into six groups,with 10 rats in each group by using UPLC-MC/MC technique.The rats in blank control group were fed with normal diet,and the other groups were fed with 2%potassium oxazinate combined with 12%yeast paste special diet.After 4 w,blank control group and model control group were intragastrically given normal saline,and low concentration fermentation solution group,medium concentration fermentation solution group and high concentration fermentation solution group were intragastrically given 20%,40%and 60%fermented Chinese medicine liquid,respectively,with the intragastrically given 16 mL/kg.The experiment lasted for 3 w.The results showed that there were 55 kinds of flavonoids in the fermentation broth,the relative content of which accounted for 15.39%.In animal experiments,compared with model control group,serum UA,BUN,Cr and TNF-αcontent of rats in high concentration fermentation liquid group were extremely decreased(P<0.01).The histopathologic sections of synovium of kidney and ankle showed that fermented Chinese medicine liquid could improve the injury of kidney tissue and ankle inflammation in rats with gouty arthritis.The study indicates that fermented Chinese medicine liquid has a certain protective effect on kidney injury caused by high uric acid,and has a certain anti-inflammatory effect on rats with gouty arthritis,and the effect of high concentration is the most obvious.

关 键 词：发酵中药 尿酸 痛风 乳酸菌 

分 类 号：S816.7[农业科学—饲料科学]