脑表达X连锁家族基因在泛癌诊断和预后预测中的价值  

Value of brain-expressed X-linked family genes in the diagnosis and prognosis prediction of pan-cancer

作　　者：杨敏 樊佳鑫 陈霖 郭建昇[2] Yang Min;Fan Jiaxin;Chen Lin;Guo Jiansheng(First Clinical Medical College,Shanxi Medical University,Taiyuan 030001,China;Department of Gastrointestinal Surgery,First Hospital of Shanxi Medical University,Taiyuan 030001,China)

机构地区：[1]山西医科大学第一临床医学院,太原030001 [2]山西医科大学第一医院胃肠外科,太原030001

出　　处：《肿瘤研究与临床》2023年第4期291-298,共8页Cancer Research and Clinic

摘　　要：目的:探讨脑表达X连锁(BEX)家族基因在泛癌中的差异性表达情况,及其在泛癌诊断和预后中的价值。方法:于2022年4月10日自加州大学圣克鲁斯分校(UCSC Xena)在线数据库下载来自癌症基因组图谱(TCGA)数据库中33种不同肿瘤的转录组测序(RNA-seq)数据、生存数据、免疫亚型、基于RNA和DNA甲基化的干细胞评分。利用R软件(V.4.2.0)limma包分析TCGA数据库中BEX家族基因的表达情况,通过Wilcox检验分析比较BEX家族基因在泛癌组织及正常组织中的差异性表达;根据BEX家族基因表达水平的中位值将泛癌患者分为高表达组和低表达组,采用Kaplan-Meier生存分析评估BEX家族基因表达与患者总生存(OS)的关系,采用Cox比例风险模型分析BEX家族基因表达对泛癌患者OS的影响并绘制森林图。根据相关指数Cor值分析BEX家族基因的表达与泛癌患者肿瘤微环境、肿瘤干细胞相关性,Spearman相关法分析胃癌组织的肿瘤微环境、肿瘤干细胞指数及BEX家族基因表达的相关性。从CellMiner数据库下载不同肿瘤细胞株RNA-seq及药物敏感性数据,分析BEX家族基因的表达与药物敏感性之间的相关性。使用Kruskal检验分析泛癌及胃癌免疫亚型与BEX家族基因表达间的相关性。结果:TCGA数据库中,BEX3在泛癌组织高表达,BEX2和BEX4在泛癌组织中度表达,BEX1和BEX5在泛癌组织相对低表达;BEX2、BEX3与BEX4在胆管癌中表达最高,BEX5在子宫内膜癌中表达最高,BEX1在浸润性乳腺癌中表达最高;相较于正常组织样本,BEX家族基因在多种肿瘤组织中的表达上调或下调(均 P<0.05);生存分析表明BEX家族基因的表达与多种癌症OS相关,BEX1、BEX3、BEX4、BEX5高表达的部分肿瘤患者OS较低表达患者好,差异均有统计学意义(均 P<0.05),其余BEX家族基因高表达患者OS较低表达患者差,差异均具有统计学意义(均 P<0.05);Cox回归分析显示,BEX1高表达对于胃癌;BEX2高表达对于急Objective To investigate the differential expression of brain-expressed X-linked(BEX)family genes in pan-cancer and its value in diagnosis and prognosis of pan-cancer.Methods RNA sequencing(RNA-seq)data,survival data,immune subtypes,the stem cell scores based on RNA and DNA methylation of 33 different tumors from The Cancer Genome Atlas(TCGA)database were downloaded from the online database of University of California,Santa Cruz(UCSC Xena)on April 10,2022.The limma package of R software(V.4.2.0)was used to analyze the expression of BEX family genes in the TCGA database.The differential expression of BEX family genes in pan-cancer tissues and normal tissues was compared by using Wilcox test.Pan-cancer patients were divided into high expression group and low expression group according to the median expression level of BEX family genes;Kaplan-Meier survival analysis was used to evaluate the relationship between the expression of BEX family genes and the overall survival(OS)of patients;Cox proportional risk model was used to analyze the effect of the expression of BEX family genes on OS in pan-cancer patients and then the forest map was drawn.The correlation of the expression of BEX family genes with tumor microenviroment and tumor stem cells in pan-cancer patients was analyzed based on the correlation index Cor value.Spearman correlation analysis was used to analyze the correlation between the expression of BEX family genes and tumor microenviroment and cancer stem cell index in gastric cancer tissues.The RNA-seq of different tumor cell lines and drug sensitivity data download from the CellMiner database were used to analyze the correlation between the expression of BEX family genes and drug sensitivity.The correlation of pan-cancer and gastric cancer immune subtypes with the expression of BEX family genes was analyzed by using Kruskal test.Results BEX3 was highly expressed in pan-cancer tissues in TCGA database,BEX2 and BEX4 were moderately expressed in pan-cancer tissues,and BEX1 and BEX5 were relatively low expre

关 键 词：基因 X连锁 泛癌 脑表达X连家族基因 生物标志物 预后 肿瘤微环境 

分 类 号：R730.43[医药卫生—肿瘤]