Transcriptome analysis of chickpea during heat stress unveils the signatures of long intergenic non-coding RNAs (lincRNAs) and mRNAs in the heat-QTL region  

作　　者：Sailaja Bhogireddy Himabindu Kudapa Prasad Bajaj Vanika Garg Annapurna Chitikineni Sourav Nayak Rajeev K.Varshney 

机构地区：[1]Center of Excellence in Genomics&Systems Biology,International Crops Research Institute for the Semi-Arid Tropics(ICRISAT),Hyderabad,India [2]Department of Horticulture,Institute of Plant Breeding,Genetics and Genomics,University of Georgia,Tifton,GA,USA [3]State Agricultural Biotechnology Centre,Centre for Crop and Food Innovation,Murdoch University,Murdoch,Western Australia,Australia [4]Nanyang Technological University,Singapore

出　　处：《Crop Design》2023年第1期29-41,共13页作物设计（英文）

基　　金：SB is thankful to Science and Engineering Research Board(SERB)-Department of Science and Technology(DST),Government of India(File no.PDF/2017/002371);RKV is thankful to SERB-DST for providing the J C Bose National Fellowship(SB/S9/Z-13/2019).

摘　　要：In plants, besides the role of messenger RNAs (mRNAs) in gene expression, long intergenic non-coding RNAs(lincRNAs) play a key role in regulating various biological processes. Chickpea (Cicer arietinum L.), an importantlegume crop, is sensitive to extreme temperature regimes. Here, we identified the lincRNAs and mRNAs in threechickpea genotypes contrasting for heat stress response (two tolerant- ICC 1356, ICC 15614;one sensitive- ICC4567) and investigated their role in heat stress. A total of 894 putative lincRNAs and 61,110 mRNAs wereidentified from leaf and root tissues at the vegetative and reproductive stages of the plant under control and heatstress conditions. Co-expression studies revealed the significant association of lincRNAs with mRNAs which areattributed to heat stress leaf samples at the reproductive stage. Further, mRNAs encoding heat shock transcriptionfactors (HSFs), heat shock proteins (HSPs), starch, and sucrose metabolism pathway genes played an essential rolein mitigating heat stress. Furthermore, three key lincRNAs underlying chickpea's heat-quantitative trait locus(QTL) region were identified. This study provided new insights into the regulation of heat stress tolerance inchickpea by identifying candidate lincRNAs and mRNAs.

关 键 词：Long non-coding RNAs MRNAS Heat stress RNA-Sequencing Heat QTL CHICKPEA 

分 类 号：S529[农业科学—作物学] Q94[生物学—植物学]