糊化制样X射线荧光光谱法快速检测鲜香菇中的铅、砷、镉  

Rapid determination of lead,arsenic and cadmium in fresh Lentinula edodes by energy-dispersive X-ray fluorescence spectrometry with gelatinizing sample preparation

作　　者：何世慧 王雨萱 王宗义[1] 刘明博 严国富 廖学亮 HE Shi-Hui;WANG Yu-Xuan;WANG Zong-Yi;LIU Ming-Bo;YAN Guo-Fu;LIAO Xue-Liang(Beijing University of Agriculture,College of Food Science and Engineering,Beijing Laboratory of Food Quality and Safety,Beijing Key Laboratory of Detection and Control of Spoilage Microorganisms and Pesticide Residues in Agricultural Products,Beijing 102206,China;NCS Testing Technology Co.,Ltd.,Beijing 100081,China;Beijing Leili Balance Agricultural Development Co.,Ltd.,Beijing 101407,China)

机构地区：[1]北京农学院食品科学与工程学院,食品质量安全北京实验室,农产品有害微生物及农残安全检测与控制北京市重点实验室,北京102206 [2]钢研纳克检测技术股份有限公司,北京100081 [3]北京雷力平衡农业发展有限公司,北京101407

出　　处：《食品安全质量检测学报》2023年第11期249-254,共6页Journal of Food Safety and Quality

基　　金：首都科技创新券资助项目(2020);北京农学院研究生科研创新项目(2022)。

摘　　要：目的建立糊化制样能量色散X射线荧光(energy-dispersive X-ray fluorescence,EDXRF)光谱法快速检测鲜香菇中铅(Pb)、砷(As)和镉(Cd)的新方法。方法鲜香菇样品与稀硝酸水溶液均质糊化后,直接进行EDXRF光扫描,通过谱峰识别进行定性,以加标校正曲线进行定量。结果样品基质中待测元素谱峰无干扰,方法线性良好(r 2≥0.995);Pb、As和Cd的检出限分别为0.34、0.14和0.19 mg/kg,定量限分别为1.15、0.47和0.63 mg/kg;总体加标回收率为93.7%~105.8%,相对标准偏差为0.92%~20.60%;以食品安全限量为关注浓度,应用本方法进行合格性快速筛选,Pb、As和Cd含量在关注浓度及以上时阳性检出率为100%,在0.2 mg/kg以下时,假阳性检出率小于等于10%,符合快速检测方法的要求。结论该方法操作简单、快速和绿色无污染,可为食用菌产品的质量控制和检测提供有效的方法借鉴。Objective To develop a new method for rapid determination of lead(Pb),arsenic(As)and cadmium(Cd)in fresh Lentinula edodes(L.edodes)by energy-dispersive X-ray fluorescence(EDXRF)spectrometry with gelatinizing sample preparation.Methods The fresh L.edodes samples were homogenized and gelatinized with the dilute nitric acid aqueous solution,and then EDXRF spectrometry scanning was carried out directly.Qualitative analysis was conducted through spectral peak recognition,and quantitative analysis was conducted through calibration curve addition.Results The characteristic EDXRF peaks measured had no interference,and the method linearity was good(r 2≥0.995).The limits of detection of Pb,As and Cd were 0.34,0.14 and 0.19 mg/kg,and the limits of quantification were 1.15,0.47 and 0.63 mg/kg,respectively.The overall recoveries were 93.7%‒105.8%,and the relative standard deviations were 0.92%‒20.60%.With the food safety limits of targets elements as the concentration of concern,this method was used to carry out the qualified fast screening.The positive detection rates of As,Pb and Cd content were 100%at the concentration of concern and above,and the false positive detection rate was less than or equal to 10%when the concentration was below 0.2 mg/kg,which met the requirements of the fast detection method.Conclusion This method is simple,fast,green and pollution-free,and can provide an effective reference for the quality control and detection of edible mushroom products.

关 键 词：能量色散X射线荧光 糊化制样 铅 砷 镉 鲜香菇 快速检测 

分 类 号：TS219[轻工技术与工程—粮食、油脂及植物蛋白工程] O657.34[轻工技术与工程—食品科学与工程]