一种榆黄蘑菌丝体多糖的结构表征及其降血糖作用  

Structural Characterization and Hypoglycemic Effect of a Polysaccharide from Pleurotus citrinopileatus Mycelia

作　　者：毛美林 邓子言 李万晴 蔡长君 涂康 王誉 张宝翠 朱希强 姜宁 刘晓鹏[1,2] MAO Meilin;DENG Ziyan;LI Wanqing;CAI Changjun;TU Kang;WANG Yu;ZHANG Baocui;ZHU Xiqiang;JIANG Ning;LIU Xiaopeng(College of Biological and Food Engineering,Hubei Minzu University,Enshi 445000,China;Hubei Key Laboratory of Biological Resources Protection and Utilization,Enshi 445000,China)

机构地区：[1]湖北民族大学生物与食品工程学院,湖北恩施445000 [2]生物资源保护与利用湖北省重点实验室,湖北恩施445000

出　　处：《食品科学》2023年第11期115-123,共9页Food Science

基　　金：湖北省技术创新专项(2016AKB058,2017ABA139,2017AKB077);国家自然科学基金地区科学基金项目(82160713);湖北产业教授人才项目(2019029,2021067);湖北省高等学校大学生创新创业训练计划项目(S202110517036)。

摘　　要：为探究榆黄蘑(Pleurotus citrinopileatus)菌丝体多糖的结构及降血糖作用,首先从榆黄蘑菌丝体中分离纯化得到水溶性多糖(water-soluble polysaccharide from P.citrinopileatus,PCP-W),采用采用高效分子排阻色谱、红外光谱等方法表征其结构;然后建立2型糖尿病小鼠模型,灌胃PCP-W 8周后,检测小鼠空腹血糖水平、糖耐量和空腹胰岛素水平,计算定量胰岛素敏感性检查指数(quantitative insulin sensitivity check index,QUICKI);检测小鼠血脂、氧化应激水平和肝脏己糖激酶(hexokinase,HK)、丙酮酸激酶(pyruvate kinase,PK)活力。结果表明,PCP-W分子质量为5238 Da,含D-吡喃葡萄糖,单糖组成为甘露糖、核糖、鼠李糖、葡萄糖醛酸、半乳糖醛酸、葡萄糖、半乳糖和岩藻糖,其物质的量比为0.003∶0.010∶0.011∶0.015∶0.001∶0.944∶0.013∶0.001。治疗8周后,PCP-W能显著降低糖尿病小鼠空腹血糖水平,改善葡萄糖耐受,升高空腹胰岛素水平,改善QUICKI(P<0.01);PCP-W能显著改善糖尿病小鼠的血脂代谢和氧化应激水平,提高PK和HK的活力(P<0.01)。因此,PCP-W具备开发成降糖药物的潜力。In this study,the structure of a water-soluble polysaccharide named PCP-W from the P.citrinopileatus mycelia was investigated by high performance size exclusion chromatography(HPSEC)and infrared(IR)spectroscopy.To evaluate the in vivo hypoglycemic effect of PCP-W,the fasting blood glucose level,glucose tolerance and fasting insulin level in mice with type 2 diabetes were measured after gavage administration of PCP-W for eight weeks,the quantitative insulin sensitivity check index(QUICKI)was calculated,and the levels of serum lipids and oxidative stress and the activities of hepatic hexokinase(HK)and pyruvate kinase(PK)were detected.The results showed that the molecular mass of PCP-W,containing D-glucopyranose units,was 5238 Da.PCP-W was composed of mannose,ribose,rhamnose,glucuronic acid,galacturonic acid,glucose,galactose and fucose in a molar ratio of 0.003:0.010:0.011:0.015:0.001:0.944:0.013:0.001.After eight weeks of treatment with PCP-W,the fasting blood glucose level was reduced,the glucose tolerance was improved,the fasting insulin level was increased,and QUICKI was improved in diabetic mice significantly(P<0.01).In addition,lipid metabolism and oxidative stress levels were improved,and the activities of PK and HK were increased(P<0.01).Therefore,PCP-W has the potential to act as a hypoglycemic agent.

关 键 词：榆黄蘑 菌丝体 多糖 结构表征 降血糖 

分 类 号：R285.5[医药卫生—中药学]