乙酰羟基酸合酶在支链氨基酸生产中的研究进展  

Recent Advance in Acetohydroxy Acid Synthase for the Production of Branched Chain Amino Acids

作　　者：王钰盛 曾琦 江泽沅 柳羽哲 刘春雷[1] 闵伟红[1] WANG Yusheng;ZENG Qi;JIANG Zeyuan;LIU Yuzhe;LIU Chunlei;MIN Weihong(College of Food Science and Engineering,Jilin Agricultural University,Changchun 130118,China)

机构地区：[1]吉林农业大学食品科学与工程学院,吉林长春130118

出　　处：《食品科学》2023年第11期244-251,共8页Food Science

基　　金：“十四五”国家重点研发计划重点专项(2021YFD2101000,2021YFD2101002)。

摘　　要：乙酰羟基酸合酶(acetohydroxyacid synthase,AHAS)是一种硫胺素二磷酸(thiamine diphosphate,ThDP)依赖性酶,是催化支链氨基酸(branched chain amino acid,BCAA),即L-缬氨酸、L-亮氨酸、L-异亮氨酸生产的第一步关键酶。AHAS容易受到末端产物BCAA的反馈抑制,从而抑制该酶的活性并影响流向支链氨基酸的碳通量。因此,AHAS成为高产支链氨基酸的重要靶点,对AHAS的改造具有重要意义。本文介绍AHAS的结构、催化机理以及该酶在BCAA合成途径中的作用,总结对该酶催化过程的研究现状以及目前对AHAS的分子改造策略,最后提出该酶未来的研究方向以及改造策略。Acetohydroxy acid synthase(AHAS),a thiamine diphosphate(ThDP)-dependent enzyme,is the key enzyme that catalyzes the first step in the production of branched chain amino acids(BCAA),namely L-valine,L-leucine and L-isoleucine.AHAS is susceptible to feedback inhibition by the end product,BCAA,which inhibits the activity of the enzyme and affects carbon flux to BCAA.Therefore,AHAS is an important target for high-yield production of BCAA,and the modification of AHAS is of great significance.In this review,the structure,catalytic mechanism,and role of AHAS in the BCAA synthesis pathway are introduced.The current research status of the catalytic process of the enzyme and the current molecular modification strategies for AHAS are summarized.Finally,future research directions and modification strategies for the enzyme are proposed.

关 键 词：乙酰羟基酸合酶 支链氨基酸 反馈抑制 分子改造 

分 类 号：Q517[生物学—生物化学]