表没食子儿茶素没食子酸酯-香蕉脱支淀粉纳米颗粒的绿色制备及其性质  

Green Preparation and Properties of Epigallocatechin-3-gallate Loaded Debranched Banana Starch Nanoparticles

作　　者：段智颖 王申宛 艾斌凌[2] 郑丽丽[2] 郑晓燕[2] 杨旸 校导 杨劲松[1] 盛占武[2] DUAN Zhiying;WANG Shenwan;AI Binling;ZHENG Lili;ZHENG Xiaoyan;YANG Yang;XIAO Dao;YANG Jinsong;SHENG Zhanwu(College of Food Science and Engineering,Hainan University,Haikou 570228,China;Haikou Experimental Station,Chinese Academy of Tropical Agricultural Sciences,Haikou 570102,China)

机构地区：[1]海南大学食品科学与工程学院,海南海口570228 [2]中国热带农业科学院海口实验站,海南海口570102

出　　处：《食品科学》2023年第12期74-83,共10页Food Science

基　　金：海南省自然科学基金创新研究团队项目(320CXTD440);中央级公益性科研院所基本科研业务费专项(1630092022003);海南省重点研发计划项目(ZDYF2021XDNY144)。

摘　　要：以青香蕉淀粉为原料,采用酶解回生法探究不同回生温度和回生时间对脱支淀粉纳米颗粒(debranched starchnanoparticles,DBS-NPs)的粒度、微观形貌、晶体结构、官能团组成以及对表没食子儿茶素没食子酸酯(epigallocatechin-3-gallate,EGCG)负载能力的影响,并考察EGCG@DBS-NPs的稳定性、抗氧化性以及体外释放行为。结果表明:与青香蕉淀粉相比,DBS-NPs粒径从(23.4±7.3)μm减小到(208.5±2.7)nm,晶体结构由C型变为C→B或C→A型,并且相对结晶度和分子链之间的氢键作用力有所提升。EGCG在DBS-NP50-12中的载药量(4.34%)和包封率(86.89%)达到最大,二者在抗氧化活性方面表现出协同效应。EGCG@DBS-NP50-12在接近中性的环境下比酸性更加稳定,且在贮藏过程中稳定性显著优于游离EGCG。在体外释放研究中,EGCG@DBSNP50-12表现出良好的缓释效果,拟合释放结果符合一级动力学方程。本研究为淀粉纳米颗粒的制备和应用提供了一定参考依据,在构建淀粉基生物活性载体方面具有潜在价值。The effects of different temperature-time regimes of enzymatic retrogradation on the particle size,microscopic morphology,crystal structure,functional groups and epigallocatechin-3-gallate(EGCG)-loading capacity of debranched starch nanoparticles(DBS-NPs)prepared with green banana starch were investigated.Meanwhile,the stability,antioxidant properties and in vitro release behavior of EGCG@DBS-NPs were investigated.It was shown that compared with green banana starch,the particle size of DBS-NPs decreased from(23.4±7.3)μm to(208.5±2.7)nm,and the crystal structure changed from type C to C→B or C→A.Besides,the relative crystallinity and the hydrogen bonding force between molecular chains were enhanced.EGCG showed the highest loading capacity(4.34%)and encapsulation efficiency(86.89%)in DBSNP50-12,and their combination exhibited synergistic antioxidant activity.EGCG@DBS-NP50-12 was found to be more stable in a near neutral environment than in an acidic environment,and significantly more stable than free EGCG during storage.EGCG@DBS-NP50-12 exhibited excellent slow release behavior in vitro,which was fitted to a first-order kinetic equation.This study provides a reference for the preparation and application of starch nanoparticles,which has potential value in the construction of starch-based bioactive carriers.

关 键 词：香蕉淀粉 纳米颗粒 酶解回生 稳定性 抗氧化活性 释放动力学 

分 类 号：TS239[轻工技术与工程—粮食、油脂及植物蛋白工程]