4种方法测定小米直链淀粉含量的比较  

Comparison of Four Methods for the Determination of the Amylose Content in Foxtail Millet

作　　者：刘建垒 商博 邢晓婷 张东 常柳 孙辉 段晓亮 LIU Jianlei;SHANG Bo;XING Xiaoting;ZHANG Dong;CHANG Liu;SUN Hui;DUAN Xiaoliang(Institution of Grain Quality and Nutrition,Academy of National Food and Strategic Reserves Administration,Beijing 102629,China)

机构地区：[1]国家粮食和物资储备局科学研究院粮食品质营养研究所,北京102629

出　　处：《食品科学》2023年第12期217-224,共8页Food Science

基　　金：中央级公益性科研院所基本科研业务费专项(JY2103-1,ZX1927)。

摘　　要：选取全国小米主产省份的24份商品小米样品,分别用改进的GB/T 15683—2008《大米直链淀粉含量的测定》法、NY/T 2639—2014《稻米直链淀粉的测定分光光度法》、双波长法及伴刀豆球蛋白A(concanavalin A,ConA)法测定其直链淀粉的含量,确定不同方法测定小米直链淀粉含量的差异,以期建立小米直链淀粉含量测定的标准方法。结果表明,4种方法测定小米直链淀粉含量的结果相比较,GB/T 15683—2008法≈双波长法>NY/T 2639—2014法>Con A法;且NY/T 2639—2014法与GB/T 15683—2008法测定结果的相关系数最高,达到0.752(P=0)。NY/T 2639—2014测定结果的1.41倍与GB/T 15683—2008测定结果相近,可以较好实现小米直链淀粉含量的简易预测。总之,改进的GB/T 15683—2008法、双波长法及NY/T 2639—2014(×1.41)法均能准确反映小米中直链淀粉的含量,且极大地缩短了样品的测试时间。In this study,the amylose contents in 24 commercial foxtail millet samples from major production provinces in China were determined by four different methods,namely,the improved national standard method(GB/T 15683-2008),the spectrophotometric method(NY/T 2639-2014)recommended by China’s agricultural authority,the dual-wavelength method,and the concanavalin A(Con A)method.The results of these methods were in the following decreasing order:improved GB/T 15683-2008≈dual-wavelength>NY/T 2639-2014>Con A.The correlation coefficient between the results of improved GB/T 15683-2008 and those of NY/T 2639-2014 was highest,0.752(P=0),the former being about 1.41 times higher than the latter,which could allow simple prediction of the amylose content of foxtail millet.In conclusion,the improved GB/T 15683-2008 method,the dual-wavelength method and the NY/T 2639-2014(×1.41)method can be used to accurately determine the content of amylose in foxtail millet while greatly reducing sample testing time.

关 键 词：小米 直链淀粉 比色法 双波长法 伴刀豆球蛋白A 

分 类 号：TS237[轻工技术与工程—粮食、油脂及植物蛋白工程]